当前位置：
X-MOL 学术
›
Acta Trop.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Low occurrence of Bartonella in synanthropic mammals and associated ectoparasites in peri-urban areas from Central-Western and Southern Brazil.
Acta Tropica ( IF 2.1 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.actatropica.2020.105513 Luiz Ricardo Gonçalves 1 , Shimon Harrus 2 , Heitor Miraglia Herrera 3 , Ricardo Gutiérrez 2 , Daniela Pedrassani 4 , Wesley Arruda Gimenes Nantes 3 , Filipe Martins Santos 3 , Grasiela Edith de Oliveira Porfírio 5 , Wanessa Teixeira Gomes Barreto 5 , Gabriel Carvalho de Macedo 3 , William de Oliveira Assis 3 , João Bosco Vilela Campos 3 , Thiago Merighi Vieira da Silva 6 , Juliano Biolchi 4 , Keyla Carstens Marques de Sousa 2 , Yaarit Nachum-Biala 2 , Darci Moraes Barros-Battesti 6 , Rosangela Zacarias Machado 6 , Marcos Rogério André 6
Acta Tropica ( IF 2.1 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.actatropica.2020.105513 Luiz Ricardo Gonçalves 1 , Shimon Harrus 2 , Heitor Miraglia Herrera 3 , Ricardo Gutiérrez 2 , Daniela Pedrassani 4 , Wesley Arruda Gimenes Nantes 3 , Filipe Martins Santos 3 , Grasiela Edith de Oliveira Porfírio 5 , Wanessa Teixeira Gomes Barreto 5 , Gabriel Carvalho de Macedo 3 , William de Oliveira Assis 3 , João Bosco Vilela Campos 3 , Thiago Merighi Vieira da Silva 6 , Juliano Biolchi 4 , Keyla Carstens Marques de Sousa 2 , Yaarit Nachum-Biala 2 , Darci Moraes Barros-Battesti 6 , Rosangela Zacarias Machado 6 , Marcos Rogério André 6
Affiliation
|
Worldwide, Bartonella species are known to infect a wide range of mammalian and arthropod hosts, including humans. The current study aimed to investigate the prevalence of Bartonella spp. in synanthropic mammals captured in peri-urban areas from Central-Western and Southern Brazil and their ectoparasites. For this aim, 160 mammals belonging to four species, and 218 associated arthropods were sampled. DNA was extracted and subjected to different Bartonella screening assays. Additionally, blood samples from 48 small rodents were submitted to liquid BAPGM culture followed by qPCR assay and solid culture. Two out of 55 Rattus captured in Santa Catarina state were PCR-positive for Bartonella when targeting the nuoG, 16S, and ITS loci. Sequences showed high homology with Bartonella coopersplainsensis. Conversely, all 48 small rodents, 14 capybaras and 43 opossum DNA samples from animals trapped in Mato Grosso do Sul were Bartonella negative in the HRM real time PCR assays targeting the ITS locus and gltA gene. Additionally, all mammal-associated ectoparasites showed negativity results based on HRM real time PCR assays. The present study showed, for the first time, the occurrence of B. coopersplainsensis in Brazil, shedding some light on the distribution of rats-related Bartonella in South America. In addition, the majority of rodents and marsupials were negative for Bartonella spp. Since B. coopersplainsensis reservoirs - Rattus spp. - are widely dispersed around the globe, their zoonotic potential should be further investigated.
中文翻译：
在巴西中西部和南部的近郊地区，同生哺乳动物和相关外寄生物中的巴尔通体发生率较低。
在世界范围内，已知巴尔通体物种可感染多种哺乳动物和节肢动物宿主，包括人类。当前的研究旨在调查Bartonella spp的患病率。在巴西中西部和南部近郊地区捕获的同人类哺乳动物中的这种细菌及其外寄生虫。为了这个目的，对属于四个物种的160个哺乳动物和218个相关节肢动物进行了采样。提取DNA并进行不同的Bartonella筛选测定。另外，将来自48只小啮齿动物的血样进行液体BAPGM培养，然后进行qPCR测定和固体培养。当靶向nuoG，16S和ITS基因座时，在圣卡塔琳娜州捕获的55只大鼠中有2只对巴尔通体呈PCR阳性。序列显示与Bartonella coopersplainsensis高度同源。相反，所有48只小啮齿动物，在针对ITS基因座和gltA基因的HRM实时PCR检测中，被困在南马托格罗索州的动物的14个水豚和43个负鼠DNA样品为Bartonella阴性。此外，基于HRM实时PCR分析，所有与哺乳动物相关的体外寄生虫均显示阴性结果。本研究首次显示了巴西的B. coopersplainsensis的出现，为南美鼠类巴尔通体的分布提供了一些线索。此外，大多数啮齿动物和有袋动物对Bartonella spp呈阴性。自B. coopersplainsensis水库以来-Rattus spp。-广泛分布在全球各地，其人畜共患病潜力应进一步调查。
更新日期：2020-05-01
中文翻译：
在巴西中西部和南部的近郊地区，同生哺乳动物和相关外寄生物中的巴尔通体发生率较低。
在世界范围内，已知巴尔通体物种可感染多种哺乳动物和节肢动物宿主，包括人类。当前的研究旨在调查Bartonella spp的患病率。在巴西中西部和南部近郊地区捕获的同人类哺乳动物中的这种细菌及其外寄生虫。为了这个目的，对属于四个物种的160个哺乳动物和218个相关节肢动物进行了采样。提取DNA并进行不同的Bartonella筛选测定。另外，将来自48只小啮齿动物的血样进行液体BAPGM培养，然后进行qPCR测定和固体培养。当靶向nuoG，16S和ITS基因座时，在圣卡塔琳娜州捕获的55只大鼠中有2只对巴尔通体呈PCR阳性。序列显示与Bartonella coopersplainsensis高度同源。相反，所有48只小啮齿动物，在针对ITS基因座和gltA基因的HRM实时PCR检测中，被困在南马托格罗索州的动物的14个水豚和43个负鼠DNA样品为Bartonella阴性。此外，基于HRM实时PCR分析，所有与哺乳动物相关的体外寄生虫均显示阴性结果。本研究首次显示了巴西的B. coopersplainsensis的出现，为南美鼠类巴尔通体的分布提供了一些线索。此外，大多数啮齿动物和有袋动物对Bartonella spp呈阴性。自B. coopersplainsensis水库以来-Rattus spp。-广泛分布在全球各地，其人畜共患病潜力应进一步调查。
京公网安备 11010802027423号