Cyadox, a new antibacterial agent as the quinoxaline-1, 4-dioxides, has a good antibacterial and growth-promoting effect, and has the advantages of lower toxicity, adequate safety and faster absorption. Seven differential expressed genes (DEGs) induced by cyadox were screened in swine liver tissues, including Insulin-like Growth Factor-1 (IGF-1), Epidermal Growth Factor (EGF), Poly ADP-ribose polymerase (PARP), the Defender Against Apoptotic Death 1 (DAD1), Complement Component 3 (C3), Transketolase (TK) and cyadox-related novel gene (CRNG). To elucidate the signal mechanism that cyadox altered these genes expression, the time-effect relationship and signaling pathways related to 7 DEGs induced by cyadox were determined in Porcine Kidney-15 (PK-15) cells by RT-qPCR and the application of various signal pathway inhibitors. The phosphorylation levels of signal factors in PK-15 cells were detected by Western blot. The analyses demonstrated that, the mRNA expressions of 7 DEGs were significantly enhanced by cyadox mainly through the phosphoinositide 3-kinase (PI3K) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) signaling pathways in PK-15 cells. Furthermore, EGF might be the early response gene of cyadox to activate downstream signaling pathways and regulates the expression of other related genes or directly exerting biological effects. In brief, cyadox mainly regulates the expression of these 7 genes by PI3K and NF-кB signaling pathways to exert it's antibacterial and growth-promoting activity in PK-15 cells.

中文翻译：

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PK-15细胞中七个主要由PI3K和NF-кB信号通路激活的cyadox相关基因的转录调控。

Cyadox，作为一种新的抗菌剂，作为喹喔啉-1，4-二氧化物，具有良好的抗菌和促生长作用，具有毒性低，安全性高，吸收快的优点。在猪肝组织中筛选了由cyadox诱导的七个差异表达基因（DEG），包括胰岛素样生长因子-1（IGF-1），表皮生长因子（EGF），聚ADP-核糖聚合酶（PARP），抗辩者细胞凋亡死亡1（DAD1），补体成分3（C3），转酮醇酶（TK）和与子宫颈液有关的新基因（CRNG）。为了阐明cyadox改变这些基因表达的信号机制，通过RT-qPCR和各种信号的应用，确定了cydonx诱导的7 DEGs的时效关系和信号通路。途径抑制剂。Western blot检测PK-15细胞中信号因子的磷酸化水平。分析表明，cyadox显着增强了7个DEG的mRNA表达，主要是通过PK-中的磷酸肌醇3激酶（PI3K）和核因子kappa-轻链增强子激活的B细胞（NF-кB）信号传导途径。 15格。此外，EGF可能是cyadox的早期反应基因，可以激活下游信号通路并调节其他相关基因的表达或直接发挥生物学作用。简而言之，cyadox主要通过PI3K和NF-кB信号通路调节这7个基因的表达，从而在PK-15细胞中发挥其抗菌和促进生长的活性。cyadox主要通过PK-15细胞中的磷酸肌醇3-激酶（PI3K）和核因子κ-轻链增强信号传导途径（NF-кB）信号通路显着增强了7个DEG的mRNA表达。此外，EGF可能是cyadox的早期反应基因，可以激活下游信号通路并调节其他相关基因的表达或直接发挥生物学作用。简而言之，cyadox主要通过PI3K和NF-кB信号通路调节这7个基因的表达，从而在PK-15细胞中发挥其抗菌和促进生长的活性。CYADOX主要通过PK-15细胞中的磷酸肌醇3-激酶（PI3K）和核因子κ-轻链增强信号通路（NF-кB）信号通路显着增强了7种DEG的mRNA表达。此外，EGF可能是cyadox的早期反应基因，可以激活下游信号通路并调节其他相关基因的表达或直接发挥生物学作用。简而言之，cyadox主要通过PI3K和NF-кB信号通路调节这7个基因的表达，从而在PK-15细胞中发挥其抗菌和促进生长的活性。EGF可能是cyadox的早期反应基因，可以激活下游信号通路并调节其他相关基因的表达或直接发挥生物学作用。简而言之，cyadox主要通过PI3K和NF-кB信号通路调节这7个基因的表达，从而在PK-15细胞中发挥其抗菌和促进生长的活性。EGF可能是cyadox的早期反应基因，可激活下游信号通路并调节其他相关基因的表达或直接发挥生物学作用。简而言之，cyadox主要通过PI3K和NF-кB信号通路调节这7个基因的表达，从而在PK-15细胞中发挥其抗菌和促进生长的活性。