Fluorescein isothiocyanate (FITC) labelled anti-rabies virus ribonucleoprotein (RNP) antibodies can be used as immunoreagents in direct fluorescent antibody testing (dFAT) for rabies diagnoses. While in-house products are occasionally used by laboratories, most conjugates are commercial reagents. Commercial anti-RNP antibodies are only available for research purposes in Brazil, however, which contributes to the increasing use of in-house produced antibodies. Considering that conjugate quality may influence the results obtained during rabies diagnosis, we sought to analyze the performance requirements of in-house produced polyclonal anti-RNP IgG-FITC for application in dFAT. To that end, their reproducibility, diagnostic sensitivity, and specificity were evaluated. The titer of polyclonal anti-RNP IgG-FITC was initially determined and evaluated by dFAT, using central nervous system (CNS) samples of different animal species (dogs, cats, bovines, equines, bats, and non-human primates). As our main result, the polyclonal anti-RNP IgG-FITC reached a titer of 1:30/1:40 in dFAT, with 100% of diagnostic sensitivity and specificity. In terms of reproducibility, the antibodies, regardless the production batch, presented the same performances. In conclusion, the in-house produced polyclonal anti-RNP IgG-FITC proved suitable for rabies virus antigen detection by dFAT.

中文翻译：

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内部生产用于直接荧光抗体测试的多克隆抗狂犬病病毒核糖核蛋白IgG抗体的性能评估。

异硫氰酸荧光素（FITC）标记的抗狂犬病病毒核糖核蛋白（RNP）抗体可用作直接荧光抗体检测（dFAT）中的免疫试剂，用于狂犬病诊断。尽管实验室偶尔会使用内部产品，但大多数偶联物都是商业试剂。商业抗RNP抗体仅在巴西可用于研究目的，但是这有助于内部使用抗体的日益普及。考虑到结合物质量可能会影响狂犬病诊断过程中获得的结果，我们试图分析内部生产的多克隆抗RNP IgG-FITC在dFAT中的应用性能要求。为此，对它们的可重复性，诊断敏感性和特异性进行了评估。最初，使用不同动物物种（狗，猫，牛，马，蝙蝠和非人类灵长类动物）的中枢神经系统（CNS）样品，通过dFAT测定并评估了多克隆抗RNP IgG-FITC的滴度。作为我们的主要结果，多克隆抗RNP IgG-FITC在dFAT中的滴度达到1：30/1：40，诊断敏感性和特异性为100％。在可重复性方面，无论生产批次如何，抗体都具有相同的性能。总之，内部生产的多克隆抗RNP IgG-FITC被证明适用于通过dFAT检测狂犬病毒抗原。具有100％的诊断敏感性和特异性。在可重复性方面，无论生产批次如何，抗体都具有相同的性能。总之，内部生产的多克隆抗RNP IgG-FITC被证明适用于通过dFAT检测狂犬病毒抗原。具有100％的诊断敏感性和特异性。在可重复性方面，无论生产批次如何，抗体都具有相同的性能。总之，内部生产的多克隆抗RNP IgG-FITC被证明适用于通过dFAT检测狂犬病毒抗原。