The aim of the study was to determine whether the presence of astaxanthin (ASX) protects boar spermatozoa against damage related to cryopreservation. Pooled ejaculates extended in Beltsville Thawing Solution (BTS) were used. Three experiments were conducted: 1) sperm samples were pre-incubated overnight (17 °C) with ASX (0, 0.5, 5, 15 μM) prior to freezing and then frozen using cooling and thawing extenders supplemented with ASX (0, 0.5, 5, 15 μM); 2) sperm samples were treated with ASX (0, 0.5, 5, 15 μM) only during overnight pre-incubation (17 °C) prior to cryopreservation; and 3) a thawing extender was supplemented with ASX (0, 0.5, 5, 15 μM). The groups were as follows: control (C; no treatment), ASX 1 (0.5 μM), ASX 2 (5 μM) and ASX 3 (15 μM). Total (TM) and progressive (PM) motility was analyzed using CASA, while sperm viability, reactive oxygen species generation, lipid peroxidation and apoptoticlike changes were analyzed using flow cytometry. Sperm variables were evaluated prior to freezing as well as 30 and 150 min after thawing. In Experiment 1, the values of TM and sperm viability post-thaw were less in the ASX 3 than C group. In Experiment 2, there was no effect of ASX on any of the sperm variables evaluated, while in Experiment 3, apoptotic-like changes were less in the ASX 1 than C group. In conclusion, there was a subtle beneficial effect on cryopreserved boar spermatozoa after addition of ASX to thawing media.

该研究的目的是确定虾青素（ASX）的存在是否能保护公猪精子免受冷冻保存相关的损害。使用在Beltsville解冻溶液（BTS）中扩展的合并的射精。进行了三个实验：1）冷冻前将精子样品与ASX（0，0.5，5，15μM）预孵育过夜（17°C），然后使用补充了ASX（0，0.5， 5，15μM）; 2）仅在冷冻保存前的过夜预孵育（17°C）期间，用ASX（0、0.5、5、15μM）处理精子样品；3）解冻补充剂补充有ASX（0，0.5，5，15μM）。这些组如下：对照组（C；未处理），ASX 1（0.5μM），ASX 2（5μM）和ASX 3（15μM）。使用CASA分析了总（TM）和进行性（PM）运动，而精子的活力，使用流式细胞仪分析了活性氧的产生，脂质过氧化和凋亡样变化。冷冻前以及解冻后30和150分钟评估精子变量。在实验1中，ASX 3中的TM和精子活力解冻后的值低于C组。在实验2中，ASX对所评估的任何精子变量均无影响，而在实验3中，ASX 1中的凋亡样变化比C组少。总之，将ASX加入解冻培养基后，对冷冻保存的公猪精子有微妙的有益作用。在ASX 3中，TM和解冻后精子活力的值低于C组。在实验2中，ASX对所评估的任何精子变量均无影响，而在实验3中，ASX 1中的凋亡样变化小于C组。总之，在将ASX加入解冻培养基后，对冷冻保存的公猪精子有微妙的有益作用。在ASX 3中，TM和解冻后精子活力的值低于C组。在实验2中，ASX对所评估的任何精子变量均无影响，而在实验3中，ASX 1中的凋亡样变化比C组少。总之，将ASX加入解冻培养基后，对冷冻保存的公猪精子有微妙的有益作用。