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Inhibitor of apoptosis is an effective target gene for RNAi-mediated control of Colorado potato beetle, Leptinotarsa decemlineata.
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-04-29 , DOI: 10.1002/arch.21685 Wesley P F Máximo 1 , Jeffrey L Howell 1 , Kanakachari Mogilicherla 1 , Moslem Basij 1 , Shankar C R R Chereddy 1 , Subba R Palli 1
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-04-29 , DOI: 10.1002/arch.21685 Wesley P F Máximo 1 , Jeffrey L Howell 1 , Kanakachari Mogilicherla 1 , Moslem Basij 1 , Shankar C R R Chereddy 1 , Subba R Palli 1
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The Colorado potato beetle (CPB; Leptinotarsa decemlineata) is one of the most notorious and difficult to control pests of potato and other solanaceous crops in North America. This insect has evolved a remarkable ability to detoxify both plant and synthetic toxins, allowing it to feed on solanaceous plants containing toxic alkaloids and to develop resistance to synthetic chemicals used for its control. RNA interference (RNAi) is a natural mechanism that evolved as an immune response to double‐stranded RNA (dsRNA) viruses where dsRNA triggers silencing of target gene expression. RNAi is being developed as a method to control CPB. Here, we evaluated four CPB‐specific genes to identify targets for RNAi‐mediated control of this insect. Out of the four dsRNAs evaluated in CPB larvae and adults, dsIAP (dsRNA targeting inhibitor of apoptosis, iap gene) performed better than dsActin, dsHSP70, and dsDynamin in inducing larval mortality. However, in adults, the mortality induced by dsActin is significantly higher than the mortality induced by dsIAP, dsHSP70, and dsDynamin. Interestingly, a combination of dsIAP and dsActin performed better than either dsIAP or dsActin alone by inducing feeding inhibition in 24 hr and mortality in 48 hr in larvae. When the dsIAP and dsActin were expressed in the Escherichia coli HT115 strain and applied as a heat‐killed bacterial spray on potato plants, it protected the plants from CPB damage. These studies show that the combination of dsIAP and dsActin shows promise as an insecticide to control CPB.
中文翻译：
凋亡抑制剂是RNAi介导的科罗拉多马铃薯甲虫Leptinotarsa decemlineata的有效靶基因。
科罗拉多马铃薯甲虫（CPB; Leptinotarsa decemlineata）是北美最臭名昭著且最难控制的马铃薯和其他茄类作物的害虫之一。这种昆虫已经进化出了显着的能力，能够对植物毒素和合成毒素进行解毒，使其能够以含有毒性生物碱的茄科植物为食，并对用于其防治的合成化学品产生抵抗力。RNA干扰（RNAi）是一种自然机制，演变为对双链RNA（dsRNA）病毒的免疫应答，其中dsRNA触发了靶基因表达的沉默。RNAi正在开发为控制CPB的方法。在这里，我们评估了四个CPB特异性基因，以确定RNAi介导对该昆虫控制的靶标。在CPB幼虫和成虫中评估的四种dsRNA中，dsIAP（靶向凋亡抑制剂的dsRNA ，IAP基因）在诱导幼虫死亡率方面比dsActin，dsHSP70和dsDynamin更好。但是，在成年人中，dsActin诱导的死亡率明显高于dsIAP，dsHSP70和dsDynamin诱导的死亡率。有趣的是，dsIAP和dsActin的组合比单独使用dsIAP或dsActin的组合表现更好，它们能诱导幼虫在24小时内抑制进食并在48小时内致死。当dsIAP和dsActin在大肠杆菌HT115菌株中表达并作为杀热细菌喷雾剂施用到马铃薯植株上时，它可以保护植株免受CPB损害。这些研究表明，dsIAP和dsActin的结合显示出有望作为控制CPB的杀虫剂。
更新日期：2020-04-29
中文翻译：
凋亡抑制剂是RNAi介导的科罗拉多马铃薯甲虫Leptinotarsa decemlineata的有效靶基因。
科罗拉多马铃薯甲虫（CPB; Leptinotarsa decemlineata）是北美最臭名昭著且最难控制的马铃薯和其他茄类作物的害虫之一。这种昆虫已经进化出了显着的能力，能够对植物毒素和合成毒素进行解毒，使其能够以含有毒性生物碱的茄科植物为食，并对用于其防治的合成化学品产生抵抗力。RNA干扰（RNAi）是一种自然机制，演变为对双链RNA（dsRNA）病毒的免疫应答，其中dsRNA触发了靶基因表达的沉默。RNAi正在开发为控制CPB的方法。在这里，我们评估了四个CPB特异性基因，以确定RNAi介导对该昆虫控制的靶标。在CPB幼虫和成虫中评估的四种dsRNA中，dsIAP（靶向凋亡抑制剂的dsRNA ，IAP基因）在诱导幼虫死亡率方面比dsActin，dsHSP70和dsDynamin更好。但是，在成年人中，dsActin诱导的死亡率明显高于dsIAP，dsHSP70和dsDynamin诱导的死亡率。有趣的是，dsIAP和dsActin的组合比单独使用dsIAP或dsActin的组合表现更好，它们能诱导幼虫在24小时内抑制进食并在48小时内致死。当dsIAP和dsActin在大肠杆菌HT115菌株中表达并作为杀热细菌喷雾剂施用到马铃薯植株上时，它可以保护植株免受CPB损害。这些研究表明，dsIAP和dsActin的结合显示出有望作为控制CPB的杀虫剂。
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