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RNA interference-mediated control of cigarette beetle, Lasioderma serricorne.
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-04-28 , DOI: 10.1002/arch.21680 Jinmo Koo 1 , Shankar C R R Chereddy 1 , Subba R Palli 1
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-04-28 , DOI: 10.1002/arch.21680 Jinmo Koo 1 , Shankar C R R Chereddy 1 , Subba R Palli 1
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The cigarette beetle (CB; Lasioderma serricorne) is a pest on many stored products including tobacco. Fumigation is the common control method currently used. However, the options for controlling this pest are limited, due to resistance issues and phasing out of currently used chemical insecticides. Here, we evaluated RNA interference (RNAi) as a potential method for controlling the CB. RNA isolated from different stages was sequenced and assembled into a transcriptome. The CB RNA sequences showed the highest homology with those in the red flour beetle, Tribolium castaneum. Orthologs of proteins known to function in RNAi pathway were identified in the CB transcriptome, suggesting that RNAi may work well in this insect. Also, 32P‐labeled double‐stranded RNA (dsRNA) injected into CB larvae and adults was processed to small interference RNAs. We selected 12 genes that were shown to be the effective RNAi targets in T. castaneum and other insects and identified orthologs of them in the CB by searching its transcriptome. Injection of dsRNA targeting genes coding for GAWKY, Kinesin, Sec23, SNF7, and 26S proteasome subunit 6B into the CB larvae caused 100% mortality. Feeding dsRNA targeting SNF7 and 26S proteasome subunit 6B by sucrose droplet assay induced more than 90% mortality, which is 1.8 times higher than the mortality induced by dsGFP control (53%). These data demonstrate an efficient RNAi response in CB, suggesting that RNAi could be developed as an efficient method to control this pest.
中文翻译：
RNA干扰介导的香烟甲虫Lasioderma serricorne的控制。
香烟甲虫（CB; Lasioderma serricorne）是包括烟草在内的许多储存产品中的有害生物。熏蒸是当前常用的控制方法。但是，由于抗药性问题和目前正在使用的化学杀虫剂的淘汰，控制这种害虫的选择受到限制。在这里，我们评估RNA干扰（RNAi）作为控制CB的潜在方法。对从不同阶段分离出的RNA进行测序并组装成转录组。CB RNA序列与红色面粉甲虫Tribolium castaneum的同源性最高。在CB转录组中鉴定了已知在RNAi途径中起作用的蛋白质的直向同源物，这表明RNAi可能在这种昆虫中起作用。还有32注入CB幼虫和成虫中的P标记双链RNA（dsRNA）被加工成小的干扰RNA。我们选择了12个基因，这些基因被证明是锥栗和其他昆虫的有效RNAi靶标，并通过搜索其转录组在CB中鉴定了它们的直系同源基因。向CB幼虫中注射编码GAWKY，Kinesin，Sec23，SNF7和26S蛋白酶体亚基6B的dsRNA靶向基因导致100％的死亡率。喂养靶向SNF7和26S蛋白酶体亚基6B的dsRNA蔗糖滴分析法检测到的死亡率超过90％，是dsGFP对照诱导的死亡率（53％）的1.8倍。这些数据证明了CB中有效的RNAi反应，表明RNAi可以作为控制这种害虫的有效方法而开发。
更新日期：2020-04-28
中文翻译：
RNA干扰介导的香烟甲虫Lasioderma serricorne的控制。
香烟甲虫（CB; Lasioderma serricorne）是包括烟草在内的许多储存产品中的有害生物。熏蒸是当前常用的控制方法。但是，由于抗药性问题和目前正在使用的化学杀虫剂的淘汰，控制这种害虫的选择受到限制。在这里，我们评估RNA干扰（RNAi）作为控制CB的潜在方法。对从不同阶段分离出的RNA进行测序并组装成转录组。CB RNA序列与红色面粉甲虫Tribolium castaneum的同源性最高。在CB转录组中鉴定了已知在RNAi途径中起作用的蛋白质的直向同源物，这表明RNAi可能在这种昆虫中起作用。还有32注入CB幼虫和成虫中的P标记双链RNA（dsRNA）被加工成小的干扰RNA。我们选择了12个基因，这些基因被证明是锥栗和其他昆虫的有效RNAi靶标，并通过搜索其转录组在CB中鉴定了它们的直系同源基因。向CB幼虫中注射编码GAWKY，Kinesin，Sec23，SNF7和26S蛋白酶体亚基6B的dsRNA靶向基因导致100％的死亡率。喂养靶向SNF7和26S蛋白酶体亚基6B的dsRNA蔗糖滴分析法检测到的死亡率超过90％，是dsGFP对照诱导的死亡率（53％）的1.8倍。这些数据证明了CB中有效的RNAi反应，表明RNAi可以作为控制这种害虫的有效方法而开发。
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