The optimization of reproductive parameters in shrimp farming continues to be a challenge for most producing countries. Although the crustacean neuropeptides have been studied extensively in the last two decades, the functions of most of these neuropeptides remained putative. Among them, molt‐inhibiting hormone isoform II (MIH II) has shown an important role in vitellogenesis. In this study, the cDNA encoding mature MIH II peptide was isolated by RT‐PCR from the L. vannamei eyestalk. The cDNA was cloned into pET28a bacterial expression vector. Recombinant MIH II was obtained in the form of insoluble inclusion bodies and purified to ~88% purity. Two doses of rMIH II and a negative control group were assayed in vivo. The stages of ovarian maturation and spawning were recorded during 72 hr post‐injection. The results showed that ovarian maturation occurred approximately in 9% and 33% of females injected with rMIH II at the doses of 300 and 600 ng/gbw respectively. Neither maturation nor spawning was detected in the negative control group. Females injected with 600 ng/gbw, which showed vitellogenic stages III and IV, spawned. These preliminary results argue that the hormone rMIH II could be a promising candidate to induce spawning in L. vannamei shrimp.

中文翻译：

---

通过注入大肠杆菌中产生的蜕皮抑制激素同工型II诱导太平洋白虾凡纳滨对虾（Boone，1931）产卵

对大多数生产国而言，对虾养殖中繁殖参数的优化仍然是一项挑战。尽管在过去的二十年中对甲壳类神经肽进行了广泛的研究，但是大多数这些神经肽的功能仍然是假定的。其中，抑制蜕皮的激素同工型II（MIH II）在卵黄发生中发挥了重要作用。在这项研究中，通过RT-PCR从南美白对虾中分离出编码成熟MIH II肽的cDNA眼神。将该cDNA克隆到pET28a细菌表达载体中。获得了不溶性包涵体形式的重组MIH II，并将其纯化至〜88％的纯度。在体内测定了两个剂量的rMIH II和阴性对照组。在注射后72小时内记录卵巢成熟和产卵的阶段。结果表明，分别以300和600 ng / gbw的剂量注射rMIH II的女性中，约有9％和33％的女性发生卵巢成熟。在阴性对照组中未检测到成熟或产卵。注入600 ng / gbw的雌性产生卵，显示出卵黄形成阶段III和IV。这些初步结果表明，rMIH II激素可能是诱导南美白对虾虾产卵的有前途的候选物。