This work investigated the antioxidant activities of dromedary colostrum proteins before and after hydrolysis by pepsin, trypsin, α‐chymotrypsin, pancreatin and papain. The enzymatic hydrolysis affected the degrees of hydrolysis, electrophoretic profiles, molecular weight distribution and hydrophobic/hydrophilic properties of the generated peptides. The antioxidant activities were evaluated using four antioxidant assays, including 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) radical‐scavenging activities, ferric reducing power and ferrous ion chelating activity. Interestingly, the antioxidant activities of dromedary colostrum proteins were enhanced after enzymatic hydrolysis. The highest antioxidant potential was obtained by pancreatic hydrolysates (P ≤ 0.05). These results suggest that dromedary colostrum protein hydrolysates are an important source of natural antioxidant peptides.

中文翻译：

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单峰骆驼（Camelus dromedarius）初乳酶解蛋白的抗氧化活性

这项工作研究了胃蛋白酶，胰蛋白酶，α-胰凝乳蛋白酶，胰酶和木瓜蛋白酶水解前后骆驼初乳蛋白质的抗氧化活性。酶促水解影响所生成肽的水解度，电泳图谱，分子量分布和疏水/亲水性质。使用4种抗氧化剂测定法评估抗氧化活性，包括2,2-二苯基-1-吡啶并肼基（DPPH）和2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除活性，三价铁还原功率和亚铁离子螯合活性。有趣的是，酶水解后单峰初乳蛋白质的抗氧化活性增强。胰腺水解物获得最高的抗氧化潜力（P ≤0.05）。这些结果表明，单峰骆驼初乳蛋白水解物是天然抗氧化剂肽的重要来源。