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Effect of digestive enzymes on the activity of camel‐milk insulin
International Journal of Dairy Technology ( IF 2.5 ) Pub Date : 2020-01-09 , DOI: 10.1111/1471-0307.12680
Nagwa H I Abou‐Soliman 1 , Sameh Awad 2 , Marwa Mohamed Desouky 1
Affiliation  

This study investigated the effect of digestive enzymes on the activity of camel‐milk insulin. The digestion was performed using the sequential action of pepsin and pancreatin. Proteolysis degree was estimated using the O‐phthaldialdehyde method. Insulin concentration was determined using an enzyme‐linked immunosorbent assay (ELISA). Results revealed that milk proteins were partially digested by pepsin alone and the degradation was increased during the pepsin–pancreatin digestion as compared to control. Insulin lost its activity after 30 min of pepsin digestion, and it was not detected by ELISA. This study strongly suggests that insulin is not responsible for the antidiabetic action of camel's milk.

中文翻译:

消化酶对骆驼奶胰岛素活性的影响

这项研究调查了消化酶对骆驼奶胰岛素活性的影响。使用胃蛋白酶和胰酶的顺序作用进行消化。蛋白水解度是使用苯二甲醛法估算的。使用酶联免疫吸附测定(ELISA)测定胰岛素浓度。结果显示,与对照相比,仅通过胃蛋白酶可以部分消化乳蛋白,并且在胃蛋白酶-胰酶消化过程中降解程度增加。胃蛋白酶消化30分钟后,胰岛素失去活性,ELISA未检测到。这项研究强烈表明,胰岛素对骆驼奶的抗糖尿病作用不起作用。
更新日期:2020-01-09
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