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Comparative appraisal of HPLC, Chloramine‐T and Lane–Eynon methods for quantification of carbohydrates in concentrated dairy products
International Journal of Dairy Technology ( IF 2.5 ) Pub Date : 2020-04-03 , DOI: 10.1111/1471-0307.12710
Luana Cristina A da Silva 1 , José A Q Lafetá Junior 1 , Maurício O Leite 1 , Edimar A F Fontes 1 , Jane S R Coimbra 1
Affiliation  

Lactose and sucrose were quantified in sweetened condensed milk and ‘Dulce de leche’ using a high‐performance liquid chromatography (HPLC) approach as compared with the classical methods (Chloramine‐T and Lane–Eynon). Lane–Eynon method estimated the lowest values of carbohydrates for both products. The chromatographic method quantified the carbohydrate contents at the same range as Chloramine‐T method. The values of the estimated standard error (ESE) for HPLC versus Chloramine‐T (0.55–0.77 mass %) were lower than the ESE for HPLC versus Lane–Eynon (1.01–1.63 mass %). Calcium concentration in the products may explain underestimated carbohydrate contents by the Lane–Eynon method.

中文翻译:

HPLC,氯胺T和Lane-Eynon方法定量评估浓缩乳制品中碳水化合物的比较

与经典方法(Chloramine-T和Lane-Eynon)相比,采用高效液相色谱法(HPLC)对甜炼乳和'Dulce de leche'中的乳糖和蔗糖进行定量。Lane–Eynon方法估计了两种产品中最低的碳水化合物值。色谱法定量的碳水化合物含量与氯胺法相同。HPLC与氯胺-T的估计标准误(ESE)值(0.55-0.77质量%)低于HPLC与Lane-Eynon的估计标准误(1.01-1.63质量%)。产品中的钙浓度可以通过Lane-Eynon方法解释碳水化合物含量低估的原因。
更新日期:2020-04-03
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