Freeze, spray, vacuum, microwave vacuum‐ and microwave freeze‐drying were applied to Bifidobacterium animalis subsp. lactis INL1. Freeze and microwave freeze‐drying showed the highest survival after drying. When a storage test (25 °C; oxygen) was performed, these cultures were the most sensitive ones at a_w = 0.23, but the addition of lactose improved their stability after 8 weeks. Flow cytometry was useful to assess viability after drying but not during storage. Our results show that dehydration technologies other than freeze‐drying might be suitable alternatives that deserve further investigation for the preservation of sensitive probiotic bacteria. Microwave drying rendered cultures of comparable characteristics to their conventional counterparts, requiring significantly shorter drying times.

中文翻译：

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常规和非常规干燥对动物双歧杆菌亚种稳定性的影响。乳酸INL1

将冷冻，喷雾，真空，微波真空干燥和微波冷冻干燥应用于动物双歧杆菌亚种。乳酸INL1。冷冻和微波冷冻干燥显示出干燥后的最高存活率。当存储测试（25℃;氧）进行，将这些培养物在最敏感的那些一_瓦特 = 0.23，但添加乳糖可以改善8周后的稳定性。流式细胞仪可用于评估干燥后的活力，但不适用于储存过程。我们的结果表明，除冷冻干燥以外的脱水技术可能是合适的替代方法，值得进一步研究以保护敏感的益生菌。微波干燥使培养物具有与常规培养物相当的特性，因此需要明显缩短的干燥时间。