Expression of the Arcelin 1 gene from Phaseolus vulgaris L. in cowpea seeds (Vigna unguiculata L.) confers bruchid resistance,Annals of Applied Biology

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Expression of the Arcelin 1 gene from Phaseolus vulgaris L. in cowpea seeds (Vigna unguiculata L.) confers bruchid resistance
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2020-01-11 , DOI: 10.1111/aab.12568
Maria A. G. D. Grazziotin _{1,

2} , Glaucia B. Cabral ₁ , Abdulrazak B. Ibrahim ₃ , Renato B. Machado ₁ , Francisco J. L. Aragão ₁

Affiliation

Persistent decrease in the productivity of cowpea (Vigna unguiculata L.) has been partly due to attack by bruchids including Zabrotes subfasciatus and Callosobruchus maculatus. Resistance to these insects in Phaseolus vulgaris L. has been shown to be associated with arcelins, a family of seed proteins encoded by a multigenic family of lectins on the APA locus. In this work, we report the construction of an expression vector containing Arc1 gene isolated from P. vulgaris and introduced into cowpea as a strategy to confer resistance to insect attack. Following transformation and selection, feeding experiments in which C. maculatus and Z. subfasciatus were fed with transgenic (L3 and L5) and non‐transgenic (control) grains showed that introduced gene protected the transgenic line. Significant differences (p < .05 and p < .01) were found in the number of eggs laid, the number of emerging insects and the loss of grain mass in L3, compared with control, for both insects. Similar observations were made in L5 with the exception of the number of laid eggs. The strategy here described may form the basis for the development of a cowpea variety tolerant to bruchids in a crop cultivated by farmers throughout Latin America and Africa.

中文翻译：

来自菜豆的菜豆中的Arcelin 1基因在cow豆种子（Vigna unguiculata L.）中的表达赋予了对羽扇豆的抗性

of豆（Vigna unguiculata L.）的生产力持续下降，部分原因是受到包括Zabrotes subfasciatus和Callosobruchus maculatus在内的bruchids的攻击。在菜豆中对这些昆虫的抗性已显示与arcelins有关，arpelins是由APA基因座上的凝集素多基因家族编码的种子蛋白家族。在这项工作中，我们报告了一种构建的表达载体的构建，该表达载体包含从寻常疟原虫中分离并引入cow豆的Arc1基因，作为赋予对昆虫攻击抗性的策略。继转化和选择，喂养实验中，四纹豆象和筋膜梭菌被喂以转基因（L3和L5），非转基因（对照）谷物显示导入的基因保护了转基因品系。与对照相比，两种昆虫的产卵数，新出现的昆虫数和L3的粒重损失均存在显着差异（p <.05和p <.01）。在L5中进行了类似的观察，但产卵数除外。这里描述的策略可能构成了在整个拉丁美洲和非洲的农民种植的农作物中发展出能耐受布鲁氏菌的cow豆品种的基础。

更新日期：2020-01-11

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