Ethanolic crude extract prepared from autumn‐shed leaves of sugar maple (Acer saccharum Marsh.) was recently shown to have antibacterial activity against Pseudomonas cichorii and Xanthomonas campestris pv. vitians, two bacteria causing diseases in lettuce production. In this study, antibacterial activity of sugar maple autumn‐shed leaves (SMASL) extract was further investigated. SMASL ethanolic crude extract was fractionated using HPLC system and geraniin was identified as the antibacterial compound by UPLC/Q‐Tof‐MS system. Geraniin, an ellagitannin, was then purified from SMASL crude extract using a glass chromatographic C18‐reversed phase silica gel column (purification Step 1) and a semi‐preparative HPLC system equipped with 5 μm XTerra Prep MS C18 column (purification Step 2). Minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) of purified geraniin (purity of 96%) against P. cichorii and X. campestris pv. vitians were determined. X. campestris pv. vitians (MIC of 0.024 mg ml⁻¹ and MBC of 3.125 mg ml⁻¹) was more sensitive to geraniin than P. cichorii (MIC of 0.781 mg ml⁻¹ and MBC of 6.25 mg ml⁻¹). In the present study, geraniin is reported for the first time as the main antibacterial compound present in SMASL.

中文翻译：

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枫槭秋叶提取物的抗菌活性：活性化合物的鉴定

最近显示，从秋枫糖枫叶（Acer saccharum Marsh。）制备的乙醇粗提物具有抗假单胞菌（Pseudomonas cichorii）和野油菜黄单胞菌（Xanthomonas campestris pv）的抗菌活性。维提安人，两种细菌在生菜生产中引起疾病​​。在这项研究中，进一步研究了糖枫秋叶（SMASL）提取物的抗菌活性。使用HPLC系统对SMASL乙醇粗提物进行分级分离，并通过UPLC / Q‐Tof‐MS系统鉴定出香叶素为抗菌化合物。然后使用玻璃色谱C18反相硅胶色谱柱（纯化步骤1）和配备5μmXTerra Prep MS C18色谱柱的半制备HPLC系统（纯化步骤2）从SMASL粗提物中纯化出香叶菊鞣花素。纯化的香叶菊酯（P. cichorii和Campestris pv ）的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。维提安人被确定。X.campestris pv。vitians（MIC为0.024 mg ml ^-1和MBC为3.125 mg ml ^-1）比菊苣假单胞菌（MIC为0.781 mg ml ^-1和MBC 6.25 mg ml ^-1）对香叶素更敏感。在本研究中，香叶素首次报道为SMASL中的主要抗菌化合物。