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Aerogels from Native and Anionic Corn Starches Loaded with Pinhão (Araucaria angustifolia) Coat Extract: Anti‐Tumor Activity in C6 Rat Glioma Cells and In Vitro Digestibility
Starch ( IF 2.6 ) Pub Date : 2020-04-03 , DOI: 10.1002/star.201900280 Laura Martins Fonseca 1 , Francine Tavares Silva 1 , Natalia Pontes Bona 2 , Francieli Moro Stefanello 2 , Caroline Dellinghausen Borges 2 , Alvaro Renato Guerra Dias 1 , Elessandra da Rosa Zavareze 1
Starch ( IF 2.6 ) Pub Date : 2020-04-03 , DOI: 10.1002/star.201900280 Laura Martins Fonseca 1 , Francine Tavares Silva 1 , Natalia Pontes Bona 2 , Francieli Moro Stefanello 2 , Caroline Dellinghausen Borges 2 , Alvaro Renato Guerra Dias 1 , Elessandra da Rosa Zavareze 1
Affiliation
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This study aims to produce aerogels with pinhão (Araucaria angustifolia) coat extract using native and anionic starches as carrier matrix and evaluate its effect on cell viability, in vitro cytotoxicity, and in vitro digestibility using C6 rat glioma cells. The aerogels are prepared by the gelatinization of starch and several freeze/thaw cycles. The pinhão coat extract (PCE) is incorporated into the aerogels in concentrations of 5 and 10% (w/w) and evaluated for morphology. Glioma (C6) cells are exposed to free PCE at concentrations of 25, 50, 100, 200, and 300 µg mL−1 and the aerogels at 300, 700, 1000, and 2000 µg mL−1 for 48 h and 72 h. Phenolic compounds are quantified during in vitro digestion simulation. The free PCE demonstrated a significant (p < 0.05). anti‐tumor effect at the tested concentrations and time treatments. The aerogels containing 10% PCE reduce cell viability at the highest concentrations (2000 µg mL−1) in 48 and 72 h. The aerogels release 37–96% of phenolic compounds during in vitro digestion. The native and anionic starch aerogels are a highly efficient matrix with potential application as phenolic compound carriers with anti‐tumor activity.
中文翻译：
富含Pinhão（Araucaria angustifolia）大衣提取物的天然和阴离子玉米淀粉的气凝胶：C6大鼠脑胶质瘤细胞的抗肿瘤活性和体外消化率
这项研究的目的是使用天然淀粉和阴离子淀粉作为载体基质，用品红（Araucaria angustifolia）外套提取物生产气凝胶，并使用C6大鼠神经胶质瘤细胞评估其对细胞活力，体外细胞毒性和体外消化率的影响。通过淀粉的糊化和几个冷冻/融化循环来制备气凝胶。将细皮大衣提取物（PCE）以5％和10％（w / w）的浓度掺入气凝胶中，并对其形态进行评估。胶质瘤（C6）细胞暴露于浓度分别为25、50、100、200和300 µg mL -1的游离PCE中，气凝胶分别暴露于300、700、1000和2000 µg mL -1的气凝胶中48小时和72小时。在体外消化模拟过程中对酚类化合物进行定量。免费的PCE证明了p＜0.05）。在测试浓度和时间治疗下具有抗肿瘤作用。含有10％PCE的气凝胶在48和72小时内以最高浓度（2000 µg mL -1）降低细胞活力。在体外消化过程中，气凝胶释放37-96％的酚类化合物。天然和阴离子淀粉气凝胶是一种高效的基质，具有潜在的应用作为具有抗肿瘤活性的酚类化合物载体。
更新日期：2020-04-03
中文翻译：
富含Pinhão（Araucaria angustifolia）大衣提取物的天然和阴离子玉米淀粉的气凝胶：C6大鼠脑胶质瘤细胞的抗肿瘤活性和体外消化率
这项研究的目的是使用天然淀粉和阴离子淀粉作为载体基质，用品红（Araucaria angustifolia）外套提取物生产气凝胶，并使用C6大鼠神经胶质瘤细胞评估其对细胞活力，体外细胞毒性和体外消化率的影响。通过淀粉的糊化和几个冷冻/融化循环来制备气凝胶。将细皮大衣提取物（PCE）以5％和10％（w / w）的浓度掺入气凝胶中，并对其形态进行评估。胶质瘤（C6）细胞暴露于浓度分别为25、50、100、200和300 µg mL -1的游离PCE中，气凝胶分别暴露于300、700、1000和2000 µg mL -1的气凝胶中48小时和72小时。在体外消化模拟过程中对酚类化合物进行定量。免费的PCE证明了p＜0.05）。在测试浓度和时间治疗下具有抗肿瘤作用。含有10％PCE的气凝胶在48和72小时内以最高浓度（2000 µg mL -1）降低细胞活力。在体外消化过程中，气凝胶释放37-96％的酚类化合物。天然和阴离子淀粉气凝胶是一种高效的基质，具有潜在的应用作为具有抗肿瘤活性的酚类化合物载体。
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