Using Molecular Fine Structure to Identify Optimal Methods of Extracting Starch,Starch

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Using Molecular Fine Structure to Identify Optimal Methods of Extracting Starch
Starch ( IF 2.6 ) Pub Date : 2020-03-01 , DOI: 10.1002/star.201900214
Yingting Zhao _{1,

2} , Xiaoyan Tan _{1,

2} , Gaosheng Wu ₁ , Robert G. Gilbert _{1,

2}

Affiliation

Obtaining relations between starch genetics/environment, its molecular structure, and its properties requires that the extraction of starch does not extract more small than large molecules and does minimal damage to molecular structure. There is no single study examining a range of techniques with more than one starch type and with clearly defined criteria for optimal extraction. Here two very different substrates are considered: rice and chickpeas. Eight different techniques are used, and the criteria for optimal extraction are that there should be the greatest number of longer chains (quantified by the average size of the debranched starch) and of larger molecules (quantified by the average size of the whole—undebranched—molecules), these being the most susceptible to degradation and loss during extraction. Size‐exclusion chromatography is used to obtain the requisite structural data. The optimal method of extraction from rice is found by using 0.45% sodium metabisulfite at 4 °C for 0.5 h with protease addition, while that for chickpea starch extraction is by using 0.45% sodium bisulfite for 6 h and washing the starch repeatedly with water. The methodology for determining optimal methods can be readily used for other starch sources.

中文翻译：

利用分子精细结构确定提取淀粉的最佳方法

要获得淀粉遗传学/环境，其分子结构及其特性之间的关系，要求淀粉的提取不比大分子提取的小，并且对分子结构的损害最小。没有一项研究检查多种技术，其中一种以上的淀粉类型以及明确定义的最佳提取标准。这里考虑了两种截然不同的基质：大米和鹰嘴豆。使用了八种不同的技术，最佳提取的标准是，应有最大数量的较长链（以去支链淀粉的平均大小来量化）和较大分子（以全部（未分支）的平均大小来量化）分子），最容易在提取过程中降解和损失。尺寸排阻色谱法用于获得必要的结构数据。从大米中提取的最佳方法是在4°C下使用0.45％的亚硫酸氢钠添加蛋白酶0.5小时，而鹰嘴豆淀粉的提取方法是使用0.45％的亚硫酸氢钠6 h并用水反复洗涤淀粉。确定最佳方法的方法可轻松用于其他淀粉来源。

更新日期：2020-03-01

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