Astrocytes in Atp1a2-deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression.,FEBS Open Bio

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Astrocytes in Atp1a2-deficient heterozygous mice exhibit hyperactivity after induction of cortical spreading depression.
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-04-23 , DOI: 10.1002/2211-5463.12848
Hiroki Sugimoto ₁ , Masaaki Sato _{2,

3,

4} , Junichi Nakai _{2,

3} , Kiyoshi Kawakami ₁

Affiliation

The ATP1A2 coding α2 subunit of Na,K‐ATPase, which is predominantly located in astrocytes, is a causative gene of familial hemiplegic migraine type 2 (FHM2). FHM2 model mice (Atp1a2^tmCKwk/+) are susceptible to cortical spreading depression (CSD), which is profoundly related to migraine aura and headache. However, astrocytic properties during CSD have not been examined in FHM2 model mice. Using Atp1a2^tmCKwk/+ crossed with transgenic mice expressing G‐CaMP7 in cortical neurons and astrocytes (Atp1a2^+/−), we analyzed the changes in Ca²⁺ concentrations during CSD. The propagation speed of Ca²⁺ waves and the percentages of astrocytes with elevated Ca²⁺ concentrations in Atp1a2^+/− were higher than those in wild‐type mice. Increased percentages of astrocytes with elevated Ca²⁺ concentrations in Atp1a2^+/− may contribute to FHM2 pathophysiology.

中文翻译：

Atp1a2缺陷型杂合小鼠中的星形胶质细胞在诱导皮质扩散抑制后表现出过度活跃。

的ATP1A2的Na编码α2亚基，K-ATP酶，其主要位于星形胶质细胞，是家族性偏瘫性偏头痛类型2（FHM2）的致病基因。FHM2模型小鼠（Atp1a2 ^{tmCKwk / +}）易患皮层扩张抑制（CSD），这与偏头痛先兆和头痛密切相关。但是，尚未在FHM2模型小鼠中检查CSD期间的星形细胞特性。使用Atp1a2 ^{tmCKwk / +}与在皮质神经元和星形胶质细胞中表达G-CaMP7的转基因小鼠杂交（Atp1a2 ^+/-），我们分析了CSD期间Ca ²⁺浓度的变化。Ca ²⁺的传播速度Atp1a2 ^+/-中的Ca ²⁺浓度升高的星形波和百分数高于野生型小鼠。在Atp1a2 ^+/-中Ca ²⁺浓度升高的星形胶质细胞百分比增加可能有助于FHM2病理生理。

更新日期：2020-04-23

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