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MicroRNA-155-5p suppresses PD-L1 expression in lung adenocarcinoma.
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-04-22 , DOI: 10.1002/2211-5463.12853 Jiansheng Huang 1 , Qiaoyou Weng 1 , Yang Shi 1 , Weibo Mao 1 , Zhigang Zhao 1 , Rongzhen Wu 1 , Jianmin Ren 1 , Shiji Fang 1 , Chenying Lu 1 , Yongzhong Du 2 , Jiansong Ji 1
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-04-22 , DOI: 10.1002/2211-5463.12853 Jiansheng Huang 1 , Qiaoyou Weng 1 , Yang Shi 1 , Weibo Mao 1 , Zhigang Zhao 1 , Rongzhen Wu 1 , Jianmin Ren 1 , Shiji Fang 1 , Chenying Lu 1 , Yongzhong Du 2 , Jiansong Ji 1
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MiR‐155‐5p is a key oncogenic microRNA that maintains immune homeostasis and mediates cross‐talk between inflammation and tumorigenesis. High expression of programmed death ligand‐1 (PD‐L1) also plays an important role in immune tolerance in tumors. The present study aimed to explore the relationship between miR‐155‐5p and PD‐L1 in lung adenocarcinoma (LUAD) cells A549 and H1650. The expression levels of miR‐155‐5p and PD‐L1 in LUAD patients were detected by a quantitative reverse transcriptase‐polymerase chain reaction (qRT‐PCR) and mimics of miR‐155‐5p were used to model increased expression in A549 or H1650 cells. After 24 h, we measured levels of PD‐L1 by qRT‐PCR, western blotting and flow cytometry. In addition, we identified two sites in the PD‐L1 3′‐UTR (5′‐AGCAUUA‐3′ and 5′‐GCAUUAA‐3′) that can be bound by miR‐155‐5p using TargetScan (http://www.targetscan.org). Compared to normal tissue, miR‐155‐5p was overexpressed in tumor tissue (P = 0.0456), whereas the expression of PD‐L1 was not significantly different (P = 0.1349). The expression levels of miR‐155‐5p and PD‐L1 were negatively correlated (r = −0.6409, P = 0.0459 and r = −0.7544, P = 0.0117). Exogenous overexpression of miR‐155‐5p decreased the mRNA, total protein and membrane protein expression levels of PD‐L1 both in A549 and H1650 cells (P < 0.05). Taken together, our data suggest that miR‐155‐5p may suppress the expression of PD‐L1 in LUAD.
中文翻译：
MicroRNA-155-5p抑制肺腺癌中PD-L1的表达。
MiR‐155‐5p是一种关键的致癌微RNA，可维持免疫稳态并介导炎症与肿瘤发生之间的串扰。程序性死亡配体-1（PD-L1）的高表达在肿瘤的免疫耐受中也起着重要作用。本研究旨在探讨肺腺癌（LUAD）细胞A549和H1650中miR‐155-5p和PD‐L1之间的关系。通过定量逆转录酶聚合酶链反应（qRT-PCR）检测LUAD患者中miR-15.5-5p和PD-1L1的表达水平，并使用miR-15.5-5p的模拟物模拟A549或H1650中表达的增加细胞。24小时后，我们通过qRT-PCR，western印迹和流式细胞仪测量了PD-L1的水平。另外，我们在PD‐L1 3′‐UTR（5′‐AGCAUUA‐3′和5′‐GCAUUAA‐3′）中确定了两个位点，这些位点可以使用TargetScan（http：//www.targetscan.org）。与正常组织相比，miR-155-5p在肿瘤组织中过表达（P = 0.0456），而PD-L1的表达没有显着差异（P = 0.1349）。miR-155-5p和PD-L1的表达水平呈负相关（r = -0.6409，P = 0.0459和r = -0.7544，P = 0.0117）。miR-155-5p的外源性过表达降低了A549和H1650细胞中PD-L1的mRNA，总蛋白和膜蛋白表达水平（P <0.05）。综上所述，我们的数据表明miR‐155‐5p可能会抑制LUAD中PD‐L1的表达。
更新日期：2020-04-22
中文翻译：
MicroRNA-155-5p抑制肺腺癌中PD-L1的表达。
MiR‐155‐5p是一种关键的致癌微RNA，可维持免疫稳态并介导炎症与肿瘤发生之间的串扰。程序性死亡配体-1（PD-L1）的高表达在肿瘤的免疫耐受中也起着重要作用。本研究旨在探讨肺腺癌（LUAD）细胞A549和H1650中miR‐155-5p和PD‐L1之间的关系。通过定量逆转录酶聚合酶链反应（qRT-PCR）检测LUAD患者中miR-15.5-5p和PD-1L1的表达水平，并使用miR-15.5-5p的模拟物模拟A549或H1650中表达的增加细胞。24小时后，我们通过qRT-PCR，western印迹和流式细胞仪测量了PD-L1的水平。另外，我们在PD‐L1 3′‐UTR（5′‐AGCAUUA‐3′和5′‐GCAUUAA‐3′）中确定了两个位点，这些位点可以使用TargetScan（http：//www.targetscan.org）。与正常组织相比，miR-155-5p在肿瘤组织中过表达（P = 0.0456），而PD-L1的表达没有显着差异（P = 0.1349）。miR-155-5p和PD-L1的表达水平呈负相关（r = -0.6409，P = 0.0459和r = -0.7544，P = 0.0117）。miR-155-5p的外源性过表达降低了A549和H1650细胞中PD-L1的mRNA，总蛋白和膜蛋白表达水平（P <0.05）。综上所述，我们的数据表明miR‐155‐5p可能会抑制LUAD中PD‐L1的表达。
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