Corynebacterium glutamicum is well known as an important industrial amino acid producer. For a few years, its ability to produce organic acids, under micro‐aerobic or anaerobic conditions was demonstrated. This study is focused on the identification of the culture parameters influencing the organic acids production and, in particular, the succinate production, by this bacterium. Corynebacterium glutamicum 2262, used throughout this study, was a wild‐type strain, which was not genetically designed for the production of succinate. The oxygenation level and the residual glucose concentration appeared as two critical parameters for the organic acids production. The maximal succinate concentration (4.9 g L−1) corresponded to the lower kLa value of 5 h−1. Above 5 h−1, a transient accumulation of the succinate was observed. Interestingly, the stop in the succinate production was concomitant with a lower threshold glucose concentration of 9 g L−1. Taking into account this threshold, a fed‐batch culture was performed to optimize the succinate production with C. glutamicum 2262. The results showed that this wild‐type strain was able to produce 93.6 g L−1 of succinate, which is one of the highest concentration reported in the literature.

中文翻译：

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谷氨酸棒杆菌，琥珀酸的天然过量生产者？


谷氨酸棒杆菌是众所周知的重要的工业氨基酸生产者。几年来，它在微需氧或厌氧条件下产生有机酸的能力得到了证明。本研究的重点是确定影响该细菌有机酸生产，特别是琥珀酸生产的培养参数。本研究中使用的谷氨酸棒杆菌 2262 是一种野生型菌株，其基因设计并非用于生产琥珀酸。氧合水平和残余葡萄糖浓度是有机酸生产的两个关键参数。最大琥珀酸盐浓度 (4.9 g L−1) 对应于 5 h−1 的较低 kLa 值。超过 5 h−1，观察到琥珀酸盐的短暂积累。有趣的是，琥珀酸生产的停止伴随着 9 g L−1 的较低阈值葡萄糖浓度。考虑到这一阈值，进行补料分批培养以优化谷氨酸棒杆菌 2262 的琥珀酸产量。结果表明，该野生型菌株能够产生 93.6 g L−1 的琥珀酸，这是目前已知的琥珀酸产量之一。文献报道的最高浓度。