Cancer research is increasingly focused on discovering strategies to induce cancer cell apoptosis without affecting surrounding normal cells. One potential biocompatible method is mechanical vibration, which has been developed as part of the emerging field of mechanomedicine. Previous studies of mechanical vibration have employed high‐frequency vibration, which damages healthy cells. In this study, we examined the effects of brief (1 h) low‐frequency (20 Hz) mechanical vibration on glucose consumption and survival (apoptosis, necrosis, HMGB1 release) of the human epidermoid carcinoma cell line A431. We found that apoptosis, but not necrosis, was significantly increased at 48 h after mechanical vibration compared with cells maintained in static culture. In keeping with this, extracellular release of HMGB1, a necrosis marker, was lower in cultures of A431 cells subjected to mechanical vibration compared with control cells. Glucose consumption was increased in the first 24 h after mechanical vibration but returned to control levels before the onset of apoptosis. Although the precise intracellular mechanisms by which low‐frequency mechanical vibration triggers apoptosis of A431 cells is unknown, these results suggest a possible role for metabolic pathways. Mechanical vibration may thus represent a novel application of mechanomedicine to cancer therapy.

中文翻译：

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低频机械振动诱导A431表皮样癌细胞凋亡

癌症研究越来越侧重于发现在不影响周围正常细胞的情况下诱导癌细胞凋亡的策略。一种潜在的生物相容性方法是机械振动，它已被开发为新兴机械医学领域的一部分。先前对机械振动的研究采用了高频振动，这会损害健康细胞。在这项研究中，我们检查了短暂 (1 h) 低频 (20 Hz) 机械振动对人表皮样癌细胞系 A431 的葡萄糖消耗和存活（细胞凋亡、坏死、HMGB1 释放）的影响。我们发现与保持在静态培养中的细胞相比，机械振动后 48 小时细胞凋亡显着增加，但没有坏死。与此相一致，细胞外释放 HMGB1，一种坏死标志物，与对照细胞相比，受到机械振动的 A431 细胞培养物中的 机械振动后的前 24 小时葡萄糖消耗增加，但在细胞凋亡开始前恢复到控制水平。尽管低频机械振动触发 A431 细胞凋亡的精确细胞内机制尚不清楚，但这些结果表明代谢途径可能发挥作用。因此，机械振动可能代表了机械医学在癌症治疗中的新应用。尽管低频机械振动触发 A431 细胞凋亡的精确细胞内机制尚不清楚，但这些结果表明代谢途径可能发挥作用。因此，机械振动可能代表了机械医学在癌症治疗中的新应用。尽管低频机械振动触发 A431 细胞凋亡的精确细胞内机制尚不清楚，但这些结果表明代谢途径可能发挥作用。因此，机械振动可能代表了机械医学在癌症治疗中的新应用。