Sperm quality can be affected by a reduction in testicular blood flow, which can be measured by Doppler ultrasonography. The aim of this study was to correlate the Doppler velocimetry of the testicular artery with kinetics of the epididymal spermatozoa in dogs. Twenty‐two dogs (44 testicles) were evaluated by Doppler ultrasonography in five regions of the testicular artery before orchiectomy. Spermatozoa were recovered by the epididymal tail compression technique and analysed for kinetics on a computer‐assisted semen analysis (CASA system). Morphology (modified Karras) and sperm membrane integrity were analysed by eosin–nigrosine staining. Data were analysed by Pearson's correlation test (p  < .01). The mean total motility was 69.0% ± 17.7, progressive motility was 43.7% ± 14.7, average path velocity (VAP) was 127.0 µm/s ± 20.7, curvilinear velocity (VCL) was 221.0 µm/s ± 31.1, and sperm velocity index (SVI) was 389.9 ± 56.1. There were positive correlations between the peak systolic velocity (PSV) in the proximal supratesticular region with the SVI (r  = .529), VCL (r  = .555) and VAP (r  = .473), and a negative correlation with the percentage of slow spermatozoa (r  = −.463). The results suggest that the testicular artery blood flow velocity can positively affect the speed of spermatozoa movement. For the first time, we have correlated sperm kinetics with the Doppler evaluation of the testicular artery in dogs.

中文翻译：

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睾丸动脉多普勒测速仪与犬附睾精子动力学和形态特征的相关性。

精子质量会受到睾丸血流减少的影响，这可以通过多普勒超声检查来测量。这项研究的目的是使睾丸动脉的多普勒测速与犬附睾精子的动力学相关联。在睾丸切除术之前，通过多普勒超声检查对22条狗（44个睾丸）进行了多普勒超声检查。通过附睾尾部压缩技术回收精子，并在计算机辅助精液分析（CASA系统）上分析动力学。形态学（改良的Karras）和精子膜的完整性通过曙红-尼古丁染色进行了分析。数据通过Pearson相关检验（p <.01）。平均总运动度为69.0％±17.7，进行性运动度为43.7％±14.7，平均路径速度（VAP）为127.0 µm / s±20.7，曲线速度（VCL）为221.0 µm / s±31.1，精子速度指数（ SVI）为389.9±56.1。上睑球囊上端的收缩期峰值速度（PSV）与SVI（r  = .529），VCL（r  = .555）和VAP（r  = .473）之间呈正相关，与百分比之间呈负相关慢精子的数量（r  = −.463）。结果表明，睾丸动脉血流速度可对精子运动速度产生积极影响。我们首次将精子动力学与狗睾丸动脉的多普勒评估相关联。