Efficiently accumulating germ-like stem cells from mouse postnatal ovary by in situ tissue culture.,Development, Growth & Differentiation

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Efficiently accumulating germ-like stem cells from mouse postnatal ovary by in situ tissue culture.
Development, Growth & Differentiation ( IF 1.7 ) Pub Date : 2020-03-18 , DOI: 10.1111/dgd.12656
Meizi Zhang ₁ , Li Liu ₁ , Xiaomin Cao ₁ , Ye Liu ₁ , Jianyong Di ₁ , Xiuying Huang ₂ , Fangzhen Sun ₂ , Weihong Huang ₂ , Fengqin Xu ₁

Affiliation

Although recent studies have revealed that germline stem cells (GSCs) exist in the mouse postnatal ovary, how to efficiently obtain GSCs for regenerating neo-oogenesis is still a technical challenge. Here, we report that using in situ tissue culture we can efficiently accumulate large amounts of proliferating germ-like cells from mouse postnatal ovaries. Usually, more than 10,000 germ-like cells can be derived from one ovary by this method, and over 20% of these cells can grow into germ-like cells with self-renewal, which thus can serve as a good cell pool to isolate GSCs by other cell assorting methods such as FACS. This method is simple and time-saving, which should be useful for in future studies on mouse GSCs.

中文翻译：

通过原位组织培养有效地从小鼠产后卵巢中积累胚样干细胞。

尽管最近的研究表明种系干细胞（GSC）存在于小鼠出生后的卵巢中，但是如何有效地获得GSC来再生新卵子发生仍然是一项技术挑战。在这里，我们报告说，使用原位组织培养，我们可以有效地从小鼠产后卵巢中积聚大量增殖的生殖样细胞。通常，通过这种方法，可以从一个卵巢衍生出10,000多个胚样细胞，其中超过20％的细胞可以生长成具有自我更新能力的胚样细胞，因此可以作为分离GSC的良好细胞库通过其他细胞分类方法，例如FACS。此方法简单且省时，对将来对小鼠GSC的研究很有用。

更新日期：2020-03-18

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