Antibody specific to chloramphenicol (CAP) was produced from rabbit that had been immunized with CAP‐keyhole limpet hemocyanin (KLH). Using the antibodies, we established a sensitive direct competitive enzyme‐linked immunosorbent assay (dcELISA) and a gold nanoparticle immunochromatographic strip (immunostrip) for detection CAP in food samples. In the dcELISA, CAP at levels of 0.15 ng/ml causes 50% inhibition (IC₅₀) of the binding of CAP‐horseradish peroxidase to the antibodies. The overall analytical recoveries of CAP (0.25–100 ng/g) added to the honey or milk samples in the dcELISA were 81.9 and 73.7%, respectively. Onsite determination of CAP was accomplished by immunostrips with a detection limit of 0.5 ng/ml and completed within 10 min. Carefully studying 10 honey and 6 milk samples using the dcELISA and immunostrip indicated that all examined samples were negative for CAP. The presented dcELISA and immunostrip methods are sensitive enough for the rapid determination of CAP in the samples.

中文翻译：

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灵敏的酶联免疫吸附测定和金纳米颗粒免疫层析带可快速检测食品中的氯霉素

氯霉素（CAP）的特异性抗体是由用CAP匙孔戚血蓝蛋白（KLH）免疫的兔子生产的。使用这些抗体，我们建立了灵敏的直接竞争酶联免疫吸附测定（dcELISA）和金纳米颗粒免疫色谱带（immunostrip），用于检测食品样品中的CAP。在dcELISA中，CAP的浓度为0.15 ng / ml时会引起50％的抑制（IC ₅₀）CAP-辣根过氧化物酶与抗体的结合。在dcELISA中添加到蜂蜜或牛奶样品中的CAP的总分析回收率（0.25–100 ng / g）分别为81.9和73.7％。CAP的现场检测通过检测限为0.5 ng / ml的免疫条完成，并在10分钟内完成。使用dcELISA和免疫条带仔细研究了10个蜂蜜和6个牛奶样品，结果表明所有检查的样品CAP均为阴性。所提出的dcELISA和免疫条带方法足够灵敏，可以快速测定样品中的CAP。