Many laboratories sampling foods for Salmonella are interested only in presence or absence of Salmonella, so only one colony may be selected. The objectives of this study were to use two selective enrichment broths and two selective agar plating media for Salmonella recovery from naturally contaminated broiler carcass rinsates and evaluate these media combinations on Salmonella serotypes recovered from each carcass. Broiler carcasses (n = 52) from a commercial processing plant prior to chilling were rinsed with buffered peptone water and after incubation subcultured to gram‐negative and tetrathionate, and after inoculation to Rappaport Vassiliadis broth which was incubated and then streaked onto plates of Brilliant Green Sulfa and Xylose‐Lysine‐Tergitol‐4 agar. On 11/49 positive carcasses, both plating media yielded the same serotypes; for the other 38 positive samples different serotypes were found on the different plating media. Enrichment and plating media combinations influence the serotypes recovered and demonstrates bias even in a limited study such as this.

中文翻译：

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使用常规方法检测商品肉鸡屠体中多种天然存在的沙门氏菌血清型

许多采样食品沙门氏菌的实验室只对沙门氏菌的存在与否感兴趣，因此只能选择一个菌落。本研究的目的是使用两种选择性增菌肉汤和两种选择性琼脂平板培养基从自然污染的肉鸡屠体冲洗液中回收沙门氏菌，并评估这些培养基组合对从每个屠体中回收的沙门氏菌血清型。来自商业加工厂的肉鸡胴体（n = 52）在冷却前用缓冲蛋白胨水冲洗，孵育后继代培养为革兰氏阴性和连四硫酸盐，接种到 Rappaport Vassiliadis 肉汤中孵育，然后在 Brilliant Green 平板上划线Sulfa 和木糖-赖氨酸-Tergitol-4 琼脂。在 11/49 阳性屠体上，两种接种培养基产生相同的血清型；对于其他 38 个阳性样品，在不同的电镀介质上发现了不同的血清型。富集和电镀介质组合影响恢复的血清型，即使在这样的有限研究中也表现出偏差。