Salmonella has been recognized as a major foodborne pathogen for humans and animals. In this study, a multiplex real‐time recombinase polymerase amplification (RPA) was developed for simultaneous detection of Salmonella enterica serovars, Salmonella enteritidis and Salmonella typhimurium, from chicken, eggs, lettuce, and papaya. The reaction was performed for 20 min at 35°C, and the detection limit of the assay was 10² CFU/ml for pure culture. In food application, the limit of detection (LOD) of S. enteritidis and S. typhimurium using multiplex real‐time RPA without enrichment procedure was 10² CFU/25 g, respectively. After enrichment, the LOD of S. enteritidis and S. typhimurium was 10 CFU/25 g. Moreover, the result for Salmonella spp. was not significantly different from those obtained using a culture‐based method. Additionally, the assay has a lower cross‐reactivity with other pathogenic microorganisms and a good stability performance. Thus, the developed multiplex RPA assay could be used as a rapid tool for the detection of S. enteritidis and S. typhimurium in food.

中文翻译：

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使用快速，多重实时重组酶聚合酶扩增法检测食品中的肠炎沙门氏菌和鼠伤寒沙门氏菌

沙门氏菌已被认为是人类和动物的主要食源性病原体。在这项研究中，多重实时聚合酶重组酶扩增（RPA）是为同时检测开发肠道沙门氏菌血清型，沙门氏菌 肠炎和沙门 菌，鸡，鸡蛋，生菜，和木瓜。反应在35°C下进行20分钟，纯培养物的检测限为10 ² CFU / ml。在食品中的应用，检测（LOD）的极限肠炎沙门氏菌和小号。无需富集程序使用多重实时RPA的鼠伤寒为10 ²CFU / 25克。富集后，的LOD肠炎沙门氏菌和小号。鼠伤寒为10 CFU / 25 g。此外，沙门氏菌属的结果。与使用基于文化的方法获得的结果没有显着差异。此外，该测定法与其他病原微生物的交叉反应性较低，并且具有良好的稳定性能。因此，开发的多重RPA测定法可以用作检测食品中肠炎链球菌和鼠伤寒沙门氏菌的快速工具。