Recoverability of Listeria monocytogenes after coculture with Tetrahymena pyriformis,Journal of Food Safety

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Recoverability of Listeria monocytogenes after coculture with Tetrahymena pyriformis
Journal of Food Safety ( IF 1.9 ) Pub Date : 2020-02-16 , DOI: 10.1111/jfs.12778
Richard J. Meinersmann ₁ , Mark E. Berrang ₁ , Luanne L. Rigsby ₁

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Bactivory by protozoa is a major factor that limits the number of bacteria in nature and may control the presence of Listeria monocytogenes. The effectiveness of Tetrahymena pyriformis destruction of L. monocytogenes was measured. Within 1 hr, 35–40 T. pyriformis cells ingested an average of 1,219 CFU of L. monocytogenes. Gentamicin was then added to kill un‐ingested Listeria. In 24 hr, the recoverable bacteria were reduced at an exponential rate to undetectable levels (<1 per culture). A genetically diverse set of L. monocytogenes cultures all reduced Listeria recovery by the same degree. In assays without addition of gentamicin, numbers of attached L. monocytogenes cells were lessened from an average of log 6.5 CFU/2 ml culture to log 4.7 CFU/2 ml culture. T. pyriformis was capable of lowering numbers of both free‐swimming and attached L. monocytogenes. This technology may have applications to control L. monocytogenes in food processing environments.

中文翻译：

与梨形四膜虫共培养后单核细胞增生李斯特菌的恢复能力

原生动物的细菌性是限制自然界细菌数量的主要因素，并可控制单核细胞增生李斯特菌的存在。测量了梨形四膜虫破坏单核细胞增生李斯特氏菌的有效性。在 1 小时内，35–40 个梨状单核菌细胞平均摄入了 1,219 CFU 的单核细胞增生李斯特菌。然后添加庆大霉素以杀死未摄入的李斯特菌。在 24 小时内，可回收细菌以指数速率减少到无法检测到的水平（每个培养物 <1）。一组遗传多样性的单核细胞增生李斯特菌培养物都以相同的程度降低了李斯特菌的回收率。在不添加庆大霉素的测定中，附着的单核细胞增生李斯特氏菌细胞的数量从平均 log 6.5 CFU/2 ml 培养物减少到 log 4.7 CFU/2 ml 培养物。T. pyriformis 能够降低自由游泳和附着 L 的数量。单核细胞增生症。该技术可用于控制食品加工环境中的单核细胞增生李斯特氏菌。

更新日期：2020-02-16

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