This study investigated the effects of lycopene on the gene expression profile and expression of genes related to fat metabolism of Xinghua breeding hens. Seven hundred and twenty healthy breeding hens were randomly assigned to four treatments; each treatment was replicated six times with 30 hens each. Broken rice and soybean meal were adopted for the basal diet and added with 0 (control group), 20, 40 and 80 mg/kg lycopene respectively. Gene expression profile of the liver induced by lycopene and expression of genes related to fat metabolism in hens liver and intestine were analysed after 42-day feeding trial including 7-day pre-feeding period and 35-day formal period. The genes involved in fat metabolism were analysed, and we found that lycopene significantly increased the expression of PGC1α, PPARα, RXRα and RARα in the liver, PPARγ, RXRα and RXRγ in the jejunum, and RARα in the duodenum (p < .05); reduced the expression of FABP1 and FABP10 in the liver, and FATP4 in the jejunum (p < .05). By analysing gene expression profile, 158 differentially expressed genes (DEGs) including 69 up-regulated genes and 89 down-regulated genes were obtained between control group and 40 mg/kg group. KEGG pathway analysis was performed on all DEGs, and 5 pathways were obtained. In conclusion, lycopene can affect the expression of related genes, and this may be one of the reasons that lycopene can regulate fat metabolism.

中文翻译：

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番茄红素的补充调节种鸡的基因表达谱和脂肪代谢。

本研究探讨番茄红素对兴化种鸡基因表达谱及脂肪代谢相关基因表达的影响。720只健康种鸡被随机分配到四种处理中。每种处理重复六次，每组30只。基础饮食采用碎米粉和豆粕，分别添加0（对照组），20、40和80 mg / kg番茄红素。经过42天的喂养试验，包括7天的预饲期和35天的正式期，分析了番茄红素诱导的肝脏的基因表达谱以及与母鸡肝和肠中脂肪代谢相关的基因的表达。分析了与脂肪代谢有关的基因，我们发现番茄红素显着增加了肝脏中PGC1α，PPARα，RXRα和RARα，PPARγ的表达。空肠中的RXRα和RXRγ，十二指肠中的RARα（p <.05）; 降低了肝脏中FABP1和FABP10的表达以及空肠中FATP4的表达（p <.05）。通过分析基因表达谱，在对照组和40 mg / kg组之间获得了158个差异表达基因（DEG），包括69个上调基因和89个下调基因。对所有DEG进行KEGG通路分析，获得5条通路。总之，番茄红素可以影响相关基因的表达，这可能是番茄红素调节脂肪代谢的原因之一。在对照组和40 mg / kg组之间获得了158个差异表达基因（DEG），包括69个上调基因和89个下调基因。对所有DEG进行KEGG通路分析，获得5条通路。总之，番茄红素可以影响相关基因的表达，这可能是番茄红素调节脂肪代谢的原因之一。在对照组和40 mg / kg组之间获得了158个差异表达基因（DEG），包括69个上调基因和89个下调基因。对所有DEG进行KEGG通路分析，获得5条通路。总之，番茄红素可以影响相关基因的表达，这可能是番茄红素调节脂肪代谢的原因之一。