This research aims to evaluate the effects of maternal vitamin E (VE) dietary supplementation on the egg characteristics, hatchability and antioxidant status of the embryo and newly hatched chicks of prolonged storage eggs. A total of 576 75‐week‐old Ross 308 breeder hens were randomly allocated into three dietary VE treatments (100, 200 and 400 mg/kg) with 6 replicates of 32 hens, for a 12‐week feeding trial. At week 12, a total of 710 eggs were collected over a 5‐day period, and eggs per treatment were attributed into 5 replicates and stored for 14 days until incubation. The egg yolk, trunk and head of 7‐day‐old embryo and the serum, liver, brain and yolk sac of newly hatched chicks were sampled for the evaluation of antioxidant status. Results showed that as maternal dietary VE levels increased, yolk α‐tocopherol concentration increased (p < .05). Compared with 100 mg/kg VE, the use of 200 and 400 mg/kg VE increased the hatchability of set/fertile eggs and total antioxidant capacity (T‐AOC) of liver and serum in chicks (p < .05), and decreased both the early embryonic mortality and the malondialdehyde (MDA) content of trunk and head in 7‐day‐old embryos (p < .05); moreover, 400 mg/kg VE increased the yolk T‐AOC (p < .05) and decreased yolk and brain MDA content of chicks (p < .05). Brain T‐AOC of chicks in 200 mg/kg VE group was improved compared to that of chicks in 100 mg/kg VE group (p < .05). In conclusion, maternal dietary VE at 200 or 400 mg/kg could increase hatchability by decreasing early embryonic mortality and increasing the antioxidant status of egg yolk, embryo and newly hatched chicks as breeder egg storage was prolonged to 14–18 days. The suitable VE level for the broiler breeder diet was 200 mg/kg as breeder egg storage was prolonged.

中文翻译：

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母体饮食中维生素E对肉鸡种鸡长期贮藏鸡蛋的蛋性，孵化率和后代质量的影响。

这项研究旨在评估母体维生素E（VE）膳食补充剂对长期储存鸡蛋的胚胎和新孵出的雏鸡的卵特性，孵化率和抗氧化状态的影响。总共576头75周大的Ross 308种母鸡被随机分配到3种饮食VE处理（100、200和400 mg / kg）中，每组32头母鸡进行6次重复饲养，为期12周。在第5周的第12周，总共收集了710个卵，每次处理的卵被分为5个重复样本，并保存14天直至孵化。对7天大的胚胎的蛋黄，躯干和头部以及刚孵出的雏鸡的血清，肝，脑和蛋黄囊进行采样，以评估其抗氧化状态。结果表明，随着孕产妇饮食中VE水平的提高，蛋黄α-生育酚浓度也随之增加（p  <.05）。与100 mg / kg V​​E相比，200和400 mg / kg V​​E的使用增加了雏鸟的孵化率/受精卵的孵化率以及肝脏和血清的总抗氧化能力（T-AOC）（p  <.05），并且降低了7日龄胚胎的早期胚胎死亡率和躯干和头部的丙二醛（MDA）含量均是如此（p  <.05）；此外，400 mg / kg V​​E增加雏鸡的蛋黄T-AOC（p  <.05），并降低雏鸡的蛋黄和脑MDA含量（p  <.05）。与100 mg / kg V​​E组的雏鸡相比，200 mg / kg V​​E组的雏鸡的脑T-AOC有所改善（p <.05）。总之，母鸡日粮VE 200或400 mg / kg可以通过降低早期胚胎死亡率和增加蛋黄，胚胎和新孵出的雏鸡的抗氧化状态来提高孵化率，因为种鸡的卵存储时间延长到14-18天。随着种鸡蛋贮藏时间的延长，肉种鸡日粮中适宜的VE水平为200 mg / kg。