The present study was conducted with privately owned dogs and cats to investigate whether a relationship exists between the dietary AGEs and the urinary excretion of AGEs, as indication of possible effective absorption of those compounds in the intestinal tract of pet carnivores. For this purpose, data were collected from both raw fed and dry processed food (DPF) fed to dogs and cats, through spot urine sampling and questionnaires. Raw pet food (RF, low in AGE diets) was fed as a primary food source to 29 dogs and DPF to 28 dogs. Cats were categorized into 3 groups, which were RF (n = 15), DPF (n = 14) and dry and wet processed pet food (DWF, n = 25). Urinary‐free carboxymethyllysine (CML), carboxyethyllysine (CEL) and lysinoalanine (LAL) were analysed using ultrahigh‐performance liquid chromatography (UHPLC)—mass spectrometry, and were standardized for variable urine concentration by expressing the AGE concentrations as a ratio to urine creatinine (Ucr) concentration (µg/µmol Ucr). Urinary excretion of CML, CEL and LAL in dogs fed with DPF was 2.03, 2.14 and 3 times higher compared to dogs fed with RF (p < .005). Similar to the dogs, a significant difference in CML:Ucr, CEL:Ucr and LAL:Ucr between the three diet groups was observed in cats (p‐overall < 0.005, ANOVA), in which the RF fed group excreted less AGEs than the other groups. Linear regression coefficients and SE of CML:Ucr, CEL:Ucr and LAL:Ucr showed that body weight and neuter status were significantly correlated with CML and CEL excretion, but not to LAL excretion. Our results revealed a significant correlation between dietary AGEs and urinary excretion of free CML, CEL and LAL, and also showed that endogenous formation of these AGEs occurs in both dogs and cats under physiological conditions.

中文翻译：

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狗和猫晚期糖基化终产物的尿排泄

本研究是用私人拥有的狗和猫进行的，以调查饮食 AGEs 与 AGEs 尿液排泄之间是否存在关系，以表明这些化合物可能在宠物食肉动物的肠道中有效吸收。为此，通过现场尿液取样和问卷调查，从喂给狗和猫的生食和干加工食品 (DPF) 中收集数据。生宠物食品（RF，低 AGE 饮食）作为主要食物来源喂给 29 只狗，DPF 喂给 28 只狗。猫分为 3 组，分别是 RF（n  = 15）、DPF（n  = 14）和干湿加工宠物食品（DWF，n = 25)。使用超高效液相色谱 (UHPLC) 质谱法分析无尿羧甲基赖氨酸 (CML)、羧乙基赖氨酸 (CEL) 和赖氨酸丙氨酸 (LAL)，并通过将 AGE 浓度表示为尿肌酐的比率来标准化可变尿浓度(Ucr) 浓度 (µg/µmol Ucr)。与喂食 RF 的狗相比，喂食 DPF 的狗的 CML、CEL 和 LAL 的尿排泄量分别高出 2.03、2.14 和 3 倍（p <  .005）。与狗相似，在猫中观察到三个饮食组之间 CML:Ucr、CEL:Ucr 和 LAL:Ucr 的显着差异（p-总体 < 0.005，ANOVA），其中 RF 喂养组比其他组排泄更少的 AGEs。CML:Ucr、CEL:Ucr 和 LAL:Ucr 的线性回归系数和 SE 表明体重和中性状态与 CML 和 CEL 排泄显着相关，但与 LAL 排泄无关。我们的研究结果揭示了饮食 AGEs 与游离 CML、CEL 和 LAL 的尿排泄之间存在显着相关性，并且还表明这些 AGEs 的内源性形成在生理条件下发生在狗和猫身上。