Temperature is supposed to be one of the primary drivers for the bacterial diversification as well as hydrocarbon formation process of oil reservoirs. However, the bacterial community compositions are not systematically elucidated in oil reservoirs with different temperatures. Herein, the diversity of indigenous bacteria and the functional species in the water samples from oil reservoirs with different in situ temperatures was investigated by high-throughput sequencing technology. The results showed that samples in the high (65 °C) and super high (80 °C) temperature oil reservoir had significantly high bacterial richness, even more than twice as much as moderate temperature (36 °C) ones, which showed relatively high bacterial diversity. Meanwhile, the bacterial compositions were almost similar in the high temperature oil reservoirs but there were different relative abundances of the bacterial communities. Phylogenetic analysis revealed that indigenous bacteria fell into 20 phylotypes in which Proteobacteria were the principal phylum in all of samples. At the genus level, 10 out of 22 major genera displayed statistically significant differences. Among of them, Pseudomonas was extremely dominant in all of samples, while Halomonas, Caldicoprobacter, Arcobacter, and Marinobacter tended to be enriched in the high temperature oil reservoirs. Moreover, the abundance of bacterial populations exhibited important distinction in oil reservoir such as hydrocarbon-oxidizing, fermentative, nitrate-reducing, sulfate-reducing, and methanogenic bacteria. Those bacteria were strongly correlated to in situ temperature variation.

中文翻译：

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不同原位温度油藏中细菌群落和功能物种的比较分析。

温度被认为是导致油藏细菌多样化和油气形成过程的主要驱动力之一。然而，在不同温度的油藏中没有系统地阐明细菌群落组成。在本文中，通过高通量测序技术研究了原位温度不同的油藏水样中的原生细菌和功能性物种的多样性。结果表明，高温（65°C）和超高温（80°C）油藏中的样品具有很高的细菌富集度，甚至是中等温度（36°C）的两倍以上，后者显示相对较高细菌多样性。与此同时，高温油藏中的细菌组成几乎相似，但细菌群落的相对丰度却不同。系统发育分析表明，土著细菌分为20种系统型，其中细菌是所有样本中的主要系统。在属水平上，22个主要属中的10个显示出统计学上的显着差异。其中，假单胞菌在所有样品中都占绝对优势，而嗜盐单胞菌，Caldicoprobacter，Arcobacter和Marinobacter倾向于在高温油藏中富集。此外，丰富的细菌种群在油藏中表现出重要的区别，例如碳氢化合物氧化，发酵，硝酸盐还原，硫酸盐还原和产甲烷细菌。