Synechocystis strains are considered as valuable resources for production of useful metabolites. Comparative primary metabolic and lipidomic profiling of freshwater and marine Synechocystis strains is needed to understand the physiological characteristics of each strain at molecular level. In this study, we performed comparative primary metabolic and lipidomic profiling of freshwater Synechocystis sp. PCC 6803 (hereafter Synechocystis 6803) and marine Synechocystis sp. PCC 7338 (hereafter Synechocystis 7338) by using gas chromatography-mass spectrometry and nano electrospray ionization-mass spectrometry. Neophytadiene, phytol, monogalactosyldiacylglycerol (MGDG), and digalactosyldiacylglycerol (DGDG) were significantly higher in Synechocystis 6803. Levels of alanine, aspartic acid, valine, linoleic acid, linolenic acid, oleic acid, plamitic acid, stearic acid, glucosylglycerol, sucrose, phosphatidylglycerol (PG), and diacylglyceryltrimethylhomoserine (DGTS) (only detected in Synechocystis 7338) were significantly higher in Synechocystis 7338. Our results provide basic information to choose Synechocystis strains for various industrial usages, and also provide fundamental information on the future development of novel Synechocystis strains to produce high levels of specific metabolites by metabolic pathway modulation.

集胞藻菌株被认为是生产有用代谢产物的宝贵资源。需要比较淡水和海洋集胞藻菌株的主要代谢和脂质组分析，以了解每种菌株在分子水平上的生理特征。在这项研究中，我们进行了比较的主要代谢和淡水Synechocystis sp的脂质组分析。PCC 6803（以下称集藻囊藻6803）和海洋集藻囊藻sp.。PCC 7338（以下称集囊藻）7338）通过使用气相色谱-质谱和纳米电喷雾电离质谱。新藻二烯，植醇，单半乳糖基二酰基甘油（MGDG）和二半乳糖基二酰基甘油（DGDG）在集胞藻6803中显着较高。丙氨酸，天冬氨酸，缬氨酸，亚油酸，亚麻酸，油酸，松香酸，硬脂酸，硬脂酸（PG）和diacylglyceryltrimethylhomoserine（DGTS）（仅在检测到蓝藻7338）均显著高于蓝藻7338.我们的研究结果提供了基本的信息来选择蓝细菌菌株各种工业用途，并提供新颖的未来发展至关重要的信息蓝藻菌株通过代谢途径调节产生高水平的特定代谢产物。