Introduction

Monogeneans of the genus Gyrodactylus were found on the gills of specimens of the bigeye sculpin Triglops nybelini Jensen, 1944 caught by trawl in the Barents Sea in January–February 2016.

Methods

Morphological preparations of the parasites were examined and photographed under a microscope at magnifications of × 100–1000 and morphometric analyses were carried out on 22 specimens using ImageJ2 software. Eight of the specimens used for the morphological comparisons were also subjected to molecular analyses by sequencing a region of the ribosomal DNA spanning partial 18S, the internal transcribed spacers 1 and 2 (ITS1 and 2), 5.8S and partial 28S and comparing this with other species through a BlastN-search in GenBank and through phylogenetic analyses.

Results

The morphology of the species from T. nybelini was markedly different to that of any of other species of Gyrodactylus. It is characterized by having relatively long hamulus roots, a character that it shares with two other species described from marine sculpins (Cottidae); G. armatus and G. maculosi. It also has a narrow rectangular ventral bar membrane with a posterior notch which it shares with G. maculosi only. Compared with all the seven species from marine Cottidae described so far, it has the smallest opisthaptoral hard parts. A comparison of the internal transcribed spacer (ITS) rDNA sequence with available sequences in GenBank and a phylogenetic analyses also showed it to be highly divergent from other sequences. Therefore, a new species is proposed, Gyrodactylus triglopsi n. sp.

Conclusion

Both the morphological and molecular analyses support the status of G. triglopsi as a new species. This is to our knowledge the first species of Gyrodactylus described from Triglops nybelini and the description extends the list of Gyrodactylus species found on fish in the Barents Sea to 17.

中文翻译：

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球状假单胞菌 sp。（Monogenea：Gyrodactylidae）于1944年在巴伦支海的Triglops nybelini Jensen的ill（Teleostei：Cottidae）产。

介绍

2016年1月至2016年2月，在拖网捕捞的大眼石Tri Triglops nybelini Jensen（1944年）的标本found上发现了Gyrodactylus属的单种属。

方法

在显微镜下以100倍至1000倍的放大倍率检查并拍摄了寄生虫的形态制剂，并使用ImageJ2软件对22个标本进行了形态计量分析。通过对跨部分18S，内部转录间隔区1和2（ITS1和2），5.8S和部分28S的核糖体DNA区域进行测序，还对八个用于形态学比较的标本进行了分子分析，并将其与其他通过GenBank中的BlastN研究以及系统发育分析对这些物种进行了研究。

结果

从物种的形态T. nybelini是到的任何其他种类的显着不同的三代虫。它的特征是具有相对较长的ulu头根，与其他两个从海洋（Cottidae）描述的物种共有。G. armatus和G. maculosi。它也有一个狭窄的矩形腹侧条状膜，带有一个后切口，与黄斑霉菌共有。只要。与迄今为止描述的海洋七足类的所有七个物种相比，它具有最小的手足硬部分。内部转录间隔区（ITS）rDNA序列与GenBank中可用序列的比较以及系统发育分析也表明，它与其他序列高度不同。因此，提出了一个新种，Gyrodactylus triglopsi n。sp。

结论

形态学和分子分析都支持三足龙作为新物种的地位。这是第一类我们所知，三代虫从描述Triglops nybelini和描述扩展列表三代虫在巴伦支海的鱼发现17种。