The experiments were conducted for the estimation of the mitigating effect of the static magnetic field (SMF of 200 mT for 1 h) treatment on soybean under ambient UV-B stress. The SMF treated (MT) and untreated (UT) seeds were grown inside iron cages covered with polyester filters for the purpose to filter UV-A + B (< 400 nm) and UV-B (< 300 nm) radiations, polythene filter control (FC) transparent for UV (280-400 nm), and open controls (OC) were without any filters. Our results indicated that specific leaf weight, efficiency of PS II, activity of carbonic anhydrase (CA) and nitrogenase (NRA), nucleic acid and protein content, nitric oxide (NO) and yield were significantly decreased in plants of untreated seeds under UV-B stress. SMF treatment to the soybean seeds was observed to mitigate the adverse effect of ambient UV-B with a significant enhancement in above-measured parameters in plants when compared with plants of untreated seeds grown under OC/FC conditions. Chlorophyll a fluorescence transition curve (OJIP-curve) from SMF treated and UV excluded plants has shown a higher fluorescence yield especially for I–P phase as compared to the plants grown in ambient UV-B stress. Reduction in the level of superoxide anion radicle (\({\text{O}}_{2}^{{ \cdot^{ - } }}\)), hydrogen peroxide (H₂O₂), malondialdehyde (MDA) and proline content with a remarkable increase in DNA, RNA, protein and NO content, increased photosynthetic efficiency and nitrogen fixation in the leaves of soybean suggested the ameliorating effect of SMF pre-treatment against ambient UV-B induced damage. Consequently, SMF-pretreatment increased the tolerance of soybean seedlings to ambient UV-B stress as compared to the untreated seeds. The increase in carbon and nitrogen fixation ability due to SMF pre-treatment and the omission of solar UV radiation impact can be a direction for the purpose to improve the crop yield. Evaluation of the consequences of SMF treated seeds under ambient UV-B stress, and the plants from untreated seeds under solar UV exclusion indicated parallelism among the two effects.

中文翻译：

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一氧化氮和活性氧在静态磁场预处理中诱导的对大豆环境UV-B胁迫的耐受性。

进行了实验，以评估在环境UV-B胁迫下大豆的静磁场（SMF为200 mT，持续1 h）对大豆的缓解效果。经过SMF处理（MT）和未处理（UT）的种子在装有聚酯滤光片的铁笼中生长，目的是滤除UV-A + B（<400 nm）和UV-B（<300 nm）辐射，聚乙烯滤光片对照（FC）对紫外线（280-400 nm）透明，而开放式对照（OC）没有任何过滤器。我们的结果表明，未处理种子的植物在紫外线照射下比叶重，PS II效率，碳酸酐酶（CA）和固氮酶（NRA）活性，核酸和蛋白质含量，一氧化氮（NO）和产量均显着降低。 B压力。与在OC / FC条件下生长的未经处理种子的植物相比，观察到对大豆种子进行SMF处理可减轻环境UV-B的不利影响，并显着增强了上述测量参数。叶绿素与在环境UV-B胁迫下生长的植物相比，经SMF处理和不含UV的植物的荧光跃迁曲线（OJIP曲线）显示出更高的荧光产量，尤其是在I-P期。过氧化氢（H ₂ O ₂降低超氧阴离子自由基（\（{\ text {O}} _ {2} ^ {{\ cdot ^ {-}}} \））的水平），丙二醛（MDA）和脯氨酸含量以及DNA，RNA，蛋白质和NO含量的显着增加，大豆叶片中光合效率的提高和固氮作用的改善，表明SMF预处理对环境UV-B诱导的伤害有改善作用。因此，与未处理的种子相比，SMF预处理提高了大豆幼苗对环境UV-B胁迫的耐受性。SMF预处理使碳和氮固定能力的提高以及太阳紫外线辐射的影响的减少可以成为提高作物产量的方向。评估在环境UV-B胁迫下SMF处理的种子的后果以及在太阳UV排斥下未经处理的种子的植物表明两种效应之间的平行性。