The recent global climate change has directly impacted major biotic and abiotic stress factors affecting crop productivity worldwide. Therefore, the need of the hour is to develop sustainable multiple stress tolerant crops through modern biotechnological approaches to cope with climate change. Hybrid proline rich proteins (HyPRPs) are the cell-wall structural proteins, which contain an N-terminal repetitive proline-rich domain and a C-terminal conserved eight-cysteine motif domain. HyPRPs are known to regulate multiple abiotic and biotic stress responses in plants. Recently, a few HyPRPs have been characterized as negative regulators of abiotic and biotic stress responses in different plants. Disruption of such negative regulators for desirable positive phenotypic traits has been made possible through the advent of advanced genome engineering tools. In the past few years, CRISPR/Cas9 has emerged as a novel breakthrough technology for crop improvement by target specific editing of known negative regulatory host genes. Here, we have described the mechanism of action and the role of known HyPRPs in regulating different biotic and abiotic stress responses in major crop plants. We have also discussed the importance of the CRISPR/Cas9 based genome editing system in targeting known negative regulatory HyPRPs for multi-stress crop tolerance using the tomato crop model. Application of genome editing to manipulate the HyPRPs of major crop plants holds promise in developing newer stress management methods in this rapidly changing climate and would lead in the future to sustain crop productivity.

中文翻译：

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富含脯氨酸的混合蛋白 (HyPRP) 的多基因 CRISPR/Cas9 基因组编辑可实现作物可持续的多重胁迫耐受性：对一种有前途的方法的回顾。


最近的全球气候变化直接影响了影响全球作物生产力的主要生物和非生物胁迫因素。因此，当前的需要是通过现代生物技术方法开发可持续的多重胁迫耐受作物来应对气候变化。混合富含脯氨酸的蛋白（HyPRP）是细胞壁结构蛋白，其包含N端重复的富含脯氨酸结构域和C端保守的八个半胱氨酸基序结构域。 HyPRP 已知可调节植物中的多种非生物和生物胁迫反应。最近，一些 HyPRP 被认为是不同植物中非生物和生物胁迫反应的负调节因子。通过先进的基因组工程工具的出现，破坏此类负调节因子以获得所需的正表型性状成为可能。在过去的几年里，CRISPR/Cas9通过对已知的负调控宿主基因进行目标特异性编辑，已成为作物改良的一种新颖的突破性技术。在这里，我们描述了已知 HyPRP 在调节主要农作物不同生物和非生物胁迫反应中的作用机制和作用。我们还讨论了基于 CRISPR/Cas9 的基因组编辑系统在使用番茄作物模型针对已知负调控 HyPRP 来实现多胁迫作物耐受性方面的重要性。应用基因组编辑来操纵主要农作物的 HyPRP 有望在这种快速变化的气候中开发更新的胁迫管理方法，并将在未来维持农作物生产力。