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Mapping QTLs for 15 morpho-metric traits in Arabidopsis thaliana using Col-0 × Don-0 population.
Physiology and Molecular Biology of Plants ( IF 3.4 ) Pub Date : 2020-04-17 , DOI: 10.1007/s12298-020-00800-7
Astha Gupta 1, 2, 3 , Vandana Jaiswal 1 , Samir V Sawant 1, 2 , Hemant Kumar Yadav 1, 2
Affiliation  

Genome wide quantitative trait loci (QTL) mapping was conducted in Arabidopsis thaliana using F2 mapping population (Col-0 × Don-0) and SNPs markers. A total of five linkage groups were obtained with number of SNPs varying from 45 to 59 per linkage group. The composite interval mapping detected a total of 36 QTLs for 15 traits and the number of QTLs ranged from one (root length, root dry biomass, cauline leaf width, number of internodes and internode distance) to seven (for bolting days). The range of phenotypic variance explained (PVE) and logarithm of the odds ratio of these 36 QTLs was found be 0.19–38.17% and 3.0–6.26 respectively. Further, the epistatic interaction detected one main effect QTL and four epistatic QTLs. Five major QTLs viz. Qbd.nbri.4.3, Qfd.nbri.4.2, Qrdm.nbri.5.1, Qncl.nbri.2.2, Qtd.nbri.4.1 with PVE > 15.0% might be useful for fine mapping to identify genes associated with respective traits, and also for development of specialized population through marker assisted selection. The identification of additive and dominant effect QTLs and desirable alleles of each of above mentioned traits would also be important for future research.

中文翻译:

使用Col-0×Don-0种群绘制拟南芥15个形态特征的QTL。

拟南芥中使用F 2作图群体(Col-0×Don-0)和SNPs标记进行了全基因组定量性状基因座(QTL)作图。总共获得了五个连锁组,每个连锁组的SNP数量从45到59不等。复合区间作图共检测到15个性状的36个QTL,QTL的数量从一个(根长,根干生物量,茎生叶宽度,节间数和节间距离)到七个(抽for天数)不等。这36个QTL的表型变异解释范围(PVE)和比值对数的对数分别为0.19-38.17%和3.0-6.26。此外,上位性相互作用检测到一个主效应QTL和四个上位性QTL。五个主要的QTL。Qbd.nbri.4.3PVE> 15.0%的Qfd.nbri.4.2Qrdm.nbri.5.1Qncl.nbri.2.2Qtd.nbri.4.1可能有助于精细定位以鉴定与各自性状相关的基因,并有助于通过标记辅助选择。上述特征的加性和显性QTL以及理想等位基因的鉴定对于未来的研究也很重要。
更新日期:2020-04-17
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