Respiratory bacterial microbiota plays a key role in human health. Lung cancer microbiome is a significant yet an understudied area while bronchiectasis microbiome is often studied. We assessed the bacterial microbiota in the upper and lower respiratory tract of the patients with lung cancer and bronchiectasis against a healthy group and their variations in individuality. 16S rRNA gene based metagenomic sequencing was used to detect entire bacterial community along with conventional aerobic bacterial culturing. In comparison to healthy, increased bacterial diversity was observed in diseased population. Abundance of more than 1% was considered and bacteria were identified in 97% similarity. Only lung cancer patients exhibited bacteria specific to the disease: Corynebacterium tuberculostearicum and Keratinibaculum paraultunense. However, Enterococcus faecalis and Delftia tsuruhatensis were also observed limited to lung cancer and bronchiectasis respectively, in less than 1% but supported with bacterial culturing. In conclusion the disease condition and intra-group variability should be considered in future with larger cohorts to understand individual patient variability highlighting the social habits and gender of the individual.

中文翻译：

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肺癌和支气管扩张患者的细菌性细菌菌群和个体细菌变异性

呼吸细菌微生物群在人类健康中起着关键作用。肺癌微生物组是一个重要但尚未充分研究的领域，而支气管扩张微生物组则经常被研究。我们针对健康人群评估了肺癌和支气管扩张患者上呼吸道和下呼吸道的细菌菌群及其个体差异。基于16S rRNA基因的宏基因组测序用于检测整个细菌群落以及常规的有氧细菌培养。与健康人群相比，患病人群的细菌多样性增加。考虑到丰度超过1％，并且细菌的相似度为97％。仅肺癌患者表现出对该疾病具有特异性的细菌：棒状杆菌棒状杆菌和副角藻。但是，还观察到粪肠球菌和纤毛虫分别限于肺癌和支气管扩张症，少于1％，但支持细菌培养。总之，将来应与较大的队列一起考虑疾病状况和组内变异性，以了解突出患者的社会习惯和性别的个体患者变异性。