Spring orchid, Cymbidium goeringii, is one of the most important and popular species in the orchid family in Northeast Asia. The purpose of this study was to determine how closely the simple sequence repeat (SSR) genotypes match between individual plants assumed to be the same cultivar in C. goeringii. For this study, we collected 1566 samples from 17 C. goeringii cultivars of the flower-variant types and determined the frequencies and distribution of the combined genotypes of 13 SSR loci by the multiplex PCR method. The phylogenetic distance matrix and the principal coordinate analysis revealed that the correlation between horticultural phenotypes and genetic distance was loosely matched, but two cultivars of Japanese origin were clearly separated from the Korean cultivars. The results showed that the ratio of the most frequent combined genotypes in 10 cultivar groups is approximately 60%, which suggests that almost 40% of C. goeringii sold may not be genuine. This is the first forensic attempt to authenticate whether each plant is genetically genuine for the corresponding cultivars in spring orchid. We suggest preparation of a guideline for DNA deposit and profiling of the SSR genotypes for the newly registered C. goeringii cultivars and believe that this study will be helpful for establishing a molecular genetic method for forensic authentication of these cultivars.

中文翻译：

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利用SSR标记对春兰大花蕙兰花变型品种进行遗传鉴定

春兰Cymbidium goeringii是东北亚兰花科中最重要和最受欢迎的物种之一。本研究的目的是确定假设为 C. goeringii 中相同栽培品种的单个植物之间的简单序列重复 (SSR) 基因型匹配的紧密程度。在本研究中，我们从 17 个花变种类型的 C. goeringii 栽培品种中收集了 1566 个样本，并通过多重 PCR 方法确定了 13 个 SSR 基因座的组合基因型的频率和分布。系统发育距离矩阵和主坐标分析表明，园艺表型与遗传距离之间的相关性是松散匹配的，但两个日本品种与韩国品种明显分离。结果表明，10 个品种组中最常见的组合基因型的比例约为 60%，这表明出售的近 40% 的 C. goeringii 可能不是真实的。这是第一次法医尝试验证每种植物对于春季兰花中的相应品种是否具有遗传真实性。我们建议为新注册的 C. goeringii 品种制定 DNA 保藏和 SSR 基因型分析的指南，并相信这项研究将有助于建立用于这些品种法医鉴定的分子遗传方法。这是首次法医尝试验证每种植物对于春季兰花中的相应品种是否具有遗传真实性。我们建议为新注册的 C. goeringii 品种制定 DNA 保藏和 SSR 基因型分析指南，并相信这项研究将有助于建立用于这些品种法医鉴定的分子遗传方法。这是首次法医尝试验证每种植物对于春季兰花中的相应品种是否具有遗传真实性。我们建议为新注册的 C. goeringii 品种制定 DNA 保藏和 SSR 基因型分析的指南，并相信这项研究将有助于建立用于这些品种法医鉴定的分子遗传方法。