Pollen genotype selection for genes expressed in both the haploid and diploid phases of the plant life cycle can lead to correlated responses detectable in the sporophyte. Here, Impact of pollen selection for heat tolerance was assessed in segregating population of maize. Two subsets of F1 were made among which one was self-fertilized with heat treated pollen grains while other set by normal pollen where no such selection was made that resulted in two F2 populations TF2 and CF2, respectively. The TF2 population recorded significantly higher mean values for pollen per anther (4646.78 ± 176.70) than that for control F2 population (3321.89 ± 164.91). In addition, the test F2 population recorded significantly higher seed yield per plant (2.02 ± 0.72 g) than that of test F2 population (1.38 ± 0.08 g) under heat stress. These two populations were also evaluated under normal condition to verify whether the TF2 population showed heat tolerance improvement has any impact on yield attributes. The result showed non-negative impact of pollen selection on performance of TF2 under normal condition. Thus, pollen selection could be used to selectively improve the trait of interest. The segregation analysis of SSR markers followed the expected Mendelian distribution in CF2 population whereas a significant deviation in allelic frequency was observed in TF2 population. These results clearly evidence that the gametophytic selection for heat tolerance in F1 generation had positive effect towards increasing the resistant alleles in F2 population of maize.

中文翻译：

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玉米（Zea maize L.）等位基因频率的变化表明配子选择对提高耐热性的影响

在植物生命周期的单倍体和二倍体阶段表达的基因的花粉基因型选择可以导致在孢子体中可检测到的相关反应。在这里，在分离玉米种群中评估了花粉选择对耐热性的影响。制作了两个 F1 子集，其中一个用热处理花粉粒自受精，而另一个用正常花粉设置，没有进行这样的选择，分别产生了两个 F2 种群 TF2 和 CF2。TF2 群体记录的花粉平均值 (4646.78 ± 176.70) 显着高于对照 F2 群体 (3321.89 ± 164.91)。此外，在热胁迫下，测试 F2 种群的单株种子产量 (2.02 ± 0.72 g) 显着高于测试 F2 种群 (1.38 ± 0.08 g)。这两个种群也在正常条件下进行了评估，以验证 TF2 种群是否表现出耐热性改善对产量属性有任何影响。结果表明，在正常条件下，花粉选择对TF2的性能没有负面影响。因此，花粉选择可用于选择性地改善感兴趣的性状。SSR 标记的分离分析遵循 CF2 群体中预期的孟德尔分布，而在 TF2 群体中观察到等位基因频率的显着偏差。这些结果清楚地证明，在 F1 代中对耐热性的配子体选择对增加玉米 F2 群体中的抗性等位基因具有积极影响。