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Genetic dissection of Fusarium head blight resistance in spring wheat cv. ‘Glenn’
Euphytica ( IF 1.9 ) Pub Date : 2020-04-13 , DOI: 10.1007/s10681-020-02610-0
Ahmed ElFatih A. ElDoliefy , Ajay Kumar , James A. Anderson , Karl D. Glover , Sujan Mamidi , Elias M. Elias , Raed Seetan , Mohammed S. Alamri , Shahryar F. Kianian , Suraj Sapkota , Andrew Green , Mohamed Mergoum

Fusarium head blight (FHB) is a major disease of wheat in many wheat-growing regions affecting wheat yield and quality. Glenn is arguably the most FHB-resistant spring wheat cultivar in the USA. To decipher the genetics of FHB resistance in Glenn, a population of 112 recombinant inbred lines was developed from the cross Glenn × MN00261-4 and evaluated for various FHB related traits at multiple locations in two states (North Dakota and Minnesota) over 3 years. Quantitative trait loci (QTL) analysis detected 15 QTL for FHB resistance related traits and heading date (HD). One QTL for FHB incidence (INC; type I resistance), six QTL for FHB severity (SEV; type II resistance), one QTL for Fusarium damaged kernels (FDK; type IV resistance), four QTL for disease index (DI), and three QTL for HD were detected. Major QTL defined as those consistent across multiple environments and explaining > 10% of the phenotypic variation were detected on chromosomes 5BL, 6BS, and 7AS. They were associated with multiple FHB variables and HD. The stable 6BS QTL with a large effect for FHB SEV resistance corresponded to the Fhb2 gene in Sumai 3. In addition, potentially novel QTL were identified on 1AS, 5BL and 7DS. Our study showed that FHB resistance in the GM population was contributed by loci other than Fhb1 . The major QTL detected for FHB resistance have potential for use in marker-assisted breeding for FHB resistance.

中文翻译:

春小麦品种抗赤霉病的遗传分析。'格伦'

镰刀菌赤霉病(FHB)是许多小麦产区的小麦主要病害,影响小麦产量和品质。Glenn 可以说是美国最具 FHB 抗性的春小麦品种。为了破译 Glenn 中 FHB 抗性的遗传学,从 Glenn × MN00261-4 杂交中开发了 112 个重组自交系,并在 3 年内在两个州(北达科他州和明尼苏达州)的多个地点评估了各种 FHB 相关性状。数量性状位点(QTL)分析检测到15个与FHB抗性相关性状和抽穗期(HD)相关的QTL。1 个 FHB 发病率 QTL(INC;I 型抗性),6 个 FHB 严重性 QTL(SEV;II 型抗性),1 个镰刀菌损伤颗粒 QTL(FDK;IV 型抗性),4 个疾病指数(DI)QTL,以及检测到3个HD QTL。主要 QTL 定义为在多个环境中一致并解释 > 10% 的表型变异在染色体 5BL、6BS 和 7AS 上检测到的 QTL。它们与多个 FHB 变量和 HD 相关。对FHB SEV 抗性具有较大影响的稳定6BS QTL 对应于Sumai 3 中的Fhb2 基因。此外,在1AS、5BL 和7DS 上鉴定了潜在的新型QTL。我们的研究表明,转基因群体中的 FHB 抗性是由 Fhb1 以外的基因座促成的。检测到的 FHB 抗性的主要 QTL 有可能用于 FHB 抗性的标记辅助育种。对FHB SEV 抗性具有较大影响的稳定6BS QTL 对应于Sumai 3 中的Fhb2 基因。此外,在1AS、5BL 和7DS 上鉴定了潜在的新型QTL。我们的研究表明,转基因群体中的 FHB 抗性是由 Fhb1 以外的基因座促成的。检测到的 FHB 抗性的主要 QTL 有可能用于 FHB 抗性的标记辅助育种。对FHB SEV 抗性具有较大影响的稳定6BS QTL 对应于Sumai 3 中的Fhb2 基因。此外,在1AS、5BL 和7DS 上鉴定了潜在的新型QTL。我们的研究表明,转基因群体中的 FHB 抗性是由 Fhb1 以外的基因座促成的。检测到的 FHB 抗性的主要 QTL 有可能用于 FHB 抗性的标记辅助育种。
更新日期:2020-04-13
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