Artificial lighting may be an interesting opportunity for the cultivation of microalgae as an alternative to natural sunlight. In particular, light emitting diodes (LEDs) can be employed to tailor the lighting to the microalgal culture in a controlled mode in order to create flashing light. In order to establish the effect of the flashing frequency on growth and biochemical composition of a model microalga, a quasi‐isoactinic reactor, in which the light distribution is almost homogeneous, was set up. In this work, it was employed for the cultivation of the heterokont Nannochloropsis gaditana in two growth media with limiting and nonlimiting nutrients. The combined effect of nutrient concentration and flashing frequency on the growth, lipid content, fatty acid content, and pigment content was assessed for the first time. The results indicate that both nutrient concentration and flashing frequency influence the above‐mentioned parameters. In particular, under flashing light conditions, an increase of lipid content and a decrease of polyunsaturated fatty acids (PUFAs) and chlorophyll are observed when nutrients are deficient, while the opposite effects are shown when nutrients are abundant.

中文翻译：

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营养和闪烁频率的组合效应对准异光化学反应器中生长的拟南芥的生化组成变化的影响

对于代替自然阳光的微藻栽培而言，人工照明可能是一个有趣的机会。特别地，可以采用发光二极管（LED）以受控模式使照明适应微藻培养物，以产生闪光。为了确定闪烁频率对模型微藻生长和生化组成的影响，建立了一个准等光化学反应器，其光分布几乎均匀。在这项工作中，它被用来栽培杂种Nannochloropsis gaditana在具有有限和非限制性营养素的两种生长培养基中。首次评估了营养物浓度和闪蒸频率对生长，脂质含量，脂肪酸含量和色素含量的综合影响。结果表明，养分浓度和闪蒸频率均会影响上述参数。尤其是在闪光灯条件下，当营养不足时，脂质含量会增加，而多不饱和脂肪酸（PUFA）和叶绿素则会减少；而当营养丰富时，则显示相反的效果。