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Tomato T2 ribonuclease LE is involved in the response to pathogens.
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-04-30 , DOI: 10.1111/mpp.12928 Naveen Kumar Singh 1 , Einat Paz 1, 2 , Yaarit Kutsher 3 , Moshe Reuveni 3 , Amnon Lers 1
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-04-30 , DOI: 10.1111/mpp.12928 Naveen Kumar Singh 1 , Einat Paz 1, 2 , Yaarit Kutsher 3 , Moshe Reuveni 3 , Amnon Lers 1
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T2 ribonucleases (RNases) are RNA‐degrading enzymes that function in various cellular processes, mostly via RNA metabolism. T2 RNase‐encoding genes have been identified in various organisms, from bacteria to mammals, and are most diverse in plants. The existence of T2 RNase genes in almost every organism suggests an important biological function that has been conserved through evolution. In plants, T2 RNases are suggested to be involved in phosphate scavenging and recycling, and are implicated in defence responses to pathogens. We investigated the function of the tomato T2 RNase LE, known to be induced by phosphate deficiency and wounding. The possible involvement of LE in pathogen responses was examined. Expression analysis showed LE induction during fungal infection and by stimuli known to be associated with pathogen inoculation, including oxalic acid and hydrogen peroxide. Analysis of LE‐suppressed transgenic tomato lines revealed higher susceptibility to oxalic acid, a cell death‐inducing factor, compared to the wild type. This elevated sensitivity of LE‐suppressed lines was evidenced by visual signs of necrosis, and increased ion leakage and reactive oxygen species levels, indicating acceleration of cell death. Challenge of the LE‐suppressed lines with the necrotrophic pathogen Botrytis cinerea resulted in accelerated development of disease symptoms compared to the wild type, associated with suppressed expression of pathogenesis‐related marker genes. The results suggest a role for plant endogenous T2 RNases in antifungal activity.
中文翻译：
番茄T2核糖核酸酶LE参与了对病原体的反应。
T2核糖核酸酶（RNases）是RNA降解酶，主要通过RNA代谢在各种细胞过程中起作用。T2 RNase编码基因已在从细菌到哺乳动物的各种生物体中被鉴定出来，并且在植物中最为多样。几乎每个生物体中都存在T2 RNase基因，这表明其重要的生物学功能已通过进化得以保留。在植物中，T2 RNase被建议参与磷酸盐的清除和再循环，并参与对病原体的防御反应。我们调查了番茄T2 RNase LE的功能，已知它是由磷酸盐缺乏和创伤诱导的。研究了LE在病原体反应中的可能参与。表达分析表明LE真菌感染过程中的诱导和已知与病原体接种相关的刺激，包括草酸和过氧化氢。对LE抑制的转基因番茄品系的分析表明，与野生型相比，草酸对细胞死亡的诱导因子具有更高的敏感性。LE抑制系的这种敏感性的提高由坏死的视觉迹象证明，并且离子泄漏和活性氧水平增加，表明细胞死亡加速。坏死性病原体灰葡萄孢对LE抑制品系的挑战与野生型相比，可导致疾病症状的加速发展，并与发病相关标记基因的表达受到抑制有关。结果表明植物内源性T2核糖核酸酶在抗真菌活性中的作用。
更新日期：2020-04-30
中文翻译：
番茄T2核糖核酸酶LE参与了对病原体的反应。
T2核糖核酸酶（RNases）是RNA降解酶，主要通过RNA代谢在各种细胞过程中起作用。T2 RNase编码基因已在从细菌到哺乳动物的各种生物体中被鉴定出来，并且在植物中最为多样。几乎每个生物体中都存在T2 RNase基因，这表明其重要的生物学功能已通过进化得以保留。在植物中，T2 RNase被建议参与磷酸盐的清除和再循环，并参与对病原体的防御反应。我们调查了番茄T2 RNase LE的功能，已知它是由磷酸盐缺乏和创伤诱导的。研究了LE在病原体反应中的可能参与。表达分析表明LE真菌感染过程中的诱导和已知与病原体接种相关的刺激，包括草酸和过氧化氢。对LE抑制的转基因番茄品系的分析表明，与野生型相比，草酸对细胞死亡的诱导因子具有更高的敏感性。LE抑制系的这种敏感性的提高由坏死的视觉迹象证明，并且离子泄漏和活性氧水平增加，表明细胞死亡加速。坏死性病原体灰葡萄孢对LE抑制品系的挑战与野生型相比，可导致疾病症状的加速发展，并与发病相关标记基因的表达受到抑制有关。结果表明植物内源性T2核糖核酸酶在抗真菌活性中的作用。
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