当前位置: X-MOL 学术Microbiologyopen › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Plasmodium berghei Gamete Egress Protein is required for fertility of both genders.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-04-30 , DOI: 10.1002/mbo3.1038
Maria Andreadaki 1 , Tomasino Pace 2 , Felicia Grasso 2 , Inga Siden-Kiamos 1 , Stefania Mochi 2 , Leonardo Picci 2 , Lucia Bertuccini 3 , Marta Ponzi 2 , Chiara Currà 1
Affiliation  

Male and female Plasmodium gametocytes ingested by the Anopheles mosquitoes during a blood meal egress from the red blood cells by rupturing the two surrounding membranes, the parasitophorous vacuole and the red blood cell membranes. Proteins of the so‐called osmiophilic bodies, (OBs), secretory organelles resident in the cytoplasm, are important players in this process. Once gametes emerge, the female is ready to be fertilized while the male develops into motile flagellar gametes. Here, we describe the function(s) of PBANKA_1115200, which we named Gamete Egress Protein (GEP), a protein specific to malaria parasites. GEP is restricted to gametocytes, expressed in gametocytes of both genders and partly localizes to the OBs. A mutant lacking the protein shows aberrant rupture of the two surrounding membranes, while OBs discharge is delayed but not aborted. Moreover, we identified a second function of GEP during exflagellation since the axonemes of the male flagellar gametes were not motile. Genetic crossing experiments reveal that both genders are unable to establish infections in mosquitoes and thus the lack of GEP leads to a complete block in Plasmodium transmission from mice to mosquitoes. The combination of our results reveals essential and pleiotropic functions of GEP in Plasmodium gametogenesis.

中文翻译:

伯氏疟原虫配子出口蛋白是两性生育所必需的。

按蚊摄取的雄性和雌性疟原虫配子细胞进餐时蚊子通过破裂周围的两个膜,寄生虫的液泡和红细胞膜而从红细胞中流出。所谓的亲油小体(OBs)的蛋白质是驻留在细胞质中的分泌细胞器,在此过程中起着重要作用。一旦配子出现,雌性就准备受精,而雄性则发展成能运动的鞭毛配子。在这里,我们描述PBANKA_1115200的功能,我们将其命名为配子流出蛋白(GEP),这是一种针对疟疾寄生虫的蛋白。GEP限于配子细胞,在两种性别的配子细胞中均表达,并且部分定位于OB。缺少蛋白质的突变体显示出周围两个膜的异常破裂,而OB的排出被延迟但并未中止。此外,由于雄性鞭毛配子的轴突不活动,因此我们在除鞭毛期间确定了GEP的第二功能。遗传杂交实验表明,这两种性别都无法在蚊子中建立感染,因此缺乏GEP会导致蚊子完全被阻断。疟原虫从小鼠到蚊子的传播。我们的结果相结合揭示了GEP在疟原虫配子发生中的基本功能和多效性。
更新日期:2020-04-30
down
wechat
bug