2,4-Dinitrotoluene (2,4-DNT), a principal derivative generated in the synthesis of 2,4,6-trinitrotoluene, is widely used as a waterproofer, plasticizer, and gelatinizer in propellants and explosives. This compound has been documented as a priority pollutant because of its toxicity. Therefore, its removal from contaminated systems is a major focus of research and environmental attention. The presence of 2,4-DNT bacterial-degrading strains that could utilize 2,4-DNT as growth substrate in polluted sites in Ibadan, Nigeria, was determined using continual enrichment techniques on nitroaromatic mixtures. Proteus sp. strain OSES2 isolated in this study was characterized by phenotypic typing and 16S ribosomal RNA gene sequencing. Growth of the strain on 2,4-DNT resulted in an exponential increase in biomass and complete substrate utilization within 72 h, accompanied by NO3 - elimination. Degradation competence was enhanced in the presence of corn steep liquor, molasses, and Tween 80 compared with incubation without amendment. Conversely, amendment with nitrogen sources yielded no significant improvement in degradation. Use of these organic wastes as candidates in a bioremediation strategy should be exploited. This would provide a less-expensive organic source supplement for cleanup purposes, with the ultimate aim of reducing the cost of bioremediation while reducing wastes intended for landfill.

中文翻译：

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好氧降解 2,4-二硝基甲苯：原始有机废物和氮强化的影响

2,4-二硝基甲苯 (2,4-DNT) 是合成 2,4,6-三硝基甲苯的主要衍生物，广泛用作推进剂和炸药中的防水剂、增塑剂和胶凝剂。由于其毒性，该化合物已被记录为优先污染物。因此，将其从受污染的系统中去除是研究和环境关注的主要焦点。使用连续富集技术对硝基芳烃混合物进行了测定，确定存在可利用 2,4-DNT 作为生长底物的 2,4-DNT 细菌降解菌株在尼日利亚伊巴丹的污染场地。变形杆菌属 本研究中分离的 OSES2 菌株的特征在于表型分型和 16S 核糖体 RNA 基因测序。菌株在 2,4-DNT 上的生长导致生物量和底物完全利用在 72 小时内呈指数增长，伴随着 NO3 - 消除。与未添加改良剂的孵育相比，在存在玉米浆、糖蜜和吐温 80 的情况下，降解能力得到增强。相反，用氮源进行修正并没有显着改善降解。应利用这些有机废物作为生物修复策略的候选物。这将为清理目的提供更便宜的有机源补充剂，最终目的是降低生物修复的成本，同时减少用于垃圾填埋的废物。应利用这些有机废物作为生物修复策略的候选物。这将为清理目的提供更便宜的有机源补充剂，最终目的是降低生物修复的成本，同时减少用于垃圾填埋的废物。应利用这些有机废物作为生物修复策略的候选物。这将为清理目的提供更便宜的有机源补充剂，最终目的是降低生物修复的成本，同时减少用于垃圾填埋的废物。