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Quantitative proteomic analysis to determine differentially expressed proteins in axenic amastigotes of Leishmania tropica and Leishmania major
IUBMB Life ( IF 3.7 ) Pub Date : 2020-04-30 , DOI: 10.1002/iub.2300
Marzieh Ashrafmansouri 1, 2 , Nasrin Amiri-Dashatan 3 , Nayebali Ahmadi 3 , Mostafa Rezaei-Tavirani 3 , Seyyedjavad SeyyedTabaei 4 , Ali Haghighi 4
Affiliation  

Cutaneous leishmaniasis is commonly caused by Leishmania major and Leishmania tropica. In the present study, the differential expression of proteins was identified in the amastigote‐like forms of L. tropica and L. major in Iranian isolates. Initially, the samples were cultured and identified using PCR‐RFLP technique. The Leishmania isolates were then grown in host‐free (axenic) culture and prepared to amastigote‐like forms, followed by the extraction of their proteins. To identify significant differentially expressed proteins (DEPs) of two types of Leishmania, the label‐free quantitative proteomic technique was used based on sequential window acquisition of all theoretical fragment ion spectra mass spectrometry. A total of 51 up/down‐DEPs (fold change >2 and p‐value <.05) were identified between the axenic amastigote forms of L. major and L. tropica. Of these, 34 and 17 proteins were up‐regulated in L. major and L. tropica, respectively. Several enriched GO terms were identified via biological process analyses for DEPs; furthermore, the metabolic process and translation were disclosed as top category in the up‐regulated proteins of both L. major and L. tropica species. Also, the KEGG analysis revealed carbon metabolism and metabolic pathways term as the top pathways in the proteins up‐regulated in L. major and L. tropica, respectively. Taken together, the numerous novel DEPs identified between the studied species could help fully understand the molecular mechanisms of pathogenesis and provide potential drug targets and vaccine candidates.

中文翻译:

定量蛋白质组学分析以确定热带利什曼原虫和大利什曼原虫的无菌无鞭毛体中差异表达的蛋白质

皮肤利什曼病通常由大利什曼原虫和热带利什曼原虫引起。在本研究中,在伊朗分离株中 L. tropica 和 L. Major 的无鞭毛体样形式中鉴定了蛋白质的差异表达。最初,使用 PCR-RFLP 技术对样品进行培养和鉴定。然后将利什曼原虫分离物在无宿主(无菌)培养物中生长并制备成无鞭毛体样形式,然后提取其蛋白质。为了鉴定两种利什曼原虫的显着差异表达蛋白 (DEP),基于所有理论碎片离子质谱图的连续窗口采集,使用了无标记定量蛋白质组学技术。在 L. Major 和 L. 热带。其中,34 和 17 种蛋白质分别在 L. Major 和 L. tropica 中上调。通过 DEP 的生物过程分析确定了几个丰富的 GO 术语;此外,代谢过程和翻译在 L. major 和 L. tropica 物种的上调蛋白质中被公开为顶级类别。此外,KEGG 分析显示碳代谢和代谢途径分别是 L. Major 和 L. tropica 中上调蛋白质的主要途径。总之,在研究物种之间鉴定出的众多新型 DEP 有助于充分了解发病机制的分子机制,并提供潜在的药物靶点和候选疫苗。通过 DEP 的生物过程分析确定了几个丰富的 GO 术语;此外,代谢过程和翻译在 L. major 和 L. tropica 物种的上调蛋白质中被公开为顶级类别。此外,KEGG 分析显示碳代谢和代谢途径分别是 L. Major 和 L. tropica 中上调蛋白质的主要途径。总之,在研究物种之间鉴定出的众多新型 DEP 有助于充分了解发病机制的分子机制,并提供潜在的药物靶点和候选疫苗。通过 DEP 的生物过程分析确定了几个丰富的 GO 术语;此外,代谢过程和翻译在 L. major 和 L. tropica 物种的上调蛋白质中被公开为顶级类别。此外,KEGG 分析显示碳代谢和代谢途径分别是 L. Major 和 L. tropica 中上调蛋白质的主要途径。总之,在研究物种之间鉴定出的众多新型 DEP 有助于充分了解发病机制的分子机制,并提供潜在的药物靶点和候选疫苗。KEGG 分析显示碳代谢和代谢途径分别是 L. Major 和 L. tropica 中上调蛋白质的主要途径。总之,在研究物种之间鉴定出的众多新型 DEP 有助于充分了解发病机制的分子机制,并提供潜在的药物靶点和候选疫苗。KEGG 分析显示碳代谢和代谢途径分别是 L. Major 和 L. tropica 中上调蛋白质的主要途径。总之,在研究物种之间鉴定出的众多新型 DEP 有助于充分了解发病机制的分子机制,并提供潜在的药物靶点和候选疫苗。
更新日期:2020-04-30
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