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Role of autophagy in Puromycin Aminonucleoside-induced podocyte apoptosis
Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-02-26 , DOI: 10.1080/10799893.2020.1731536
Shengyou Yu 1, 2 , Qi Ren 3 , Li Yu 2 , Junjie Tan 2 , Zheng Kun Xia 1
Affiliation  

Abstract Objective: The aim of our study is to investigate the relationship between podocyte autophagy and apoptosis induced by Puromycin Aminonucleoside (PAN) and to clarify its mechanism. Methods: Podocytes were cultured in vitro. The apoptosis rates of each group were detected using flow cytometry. The expression of LC3-II protein and changes in distribution were detected through laser scanning confocal microscope, and the western blot protocol was employed for detection of protein expression of LC3-II. The autophagosomes were detected by transmission electron microscopy. Results: In this study, We found that autophagosome increased followed by apoptosis after podocyte injury. Furthermore, we conformed that the activation of autophagy could inhibit the apoptosis to alleviate the injury of podocyte at an early stage. Conclusions: Autophagy occurred earlier before apoptosis and autophagy mediated podocyte apoptosis induced by PAN. These findings indicate that autophagy may become a novel therapeutic target for the treatment of podocyte injury and proteinuria in the future.

中文翻译:

自噬在嘌呤霉素氨基核苷诱导的足细胞凋亡中的作用

摘要 目的:研究嘌呤霉素氨基核苷(PAN)诱导的足细胞自噬与细胞凋亡的关系,阐明其作用机制。方法:体外培养足细胞。流式细胞仪检测各组细胞凋亡率。激光扫描共聚焦显微镜检测LC3-II蛋白的表达及分布变化,采用Western blot方法检测LC3-II蛋白的表达。通过透射电子显微镜检测自噬体。结果:在本研究中,我们发现足细胞损伤后自噬体增加,随后凋亡。此外,我们认为自噬的激活可以抑制细胞凋亡,从而在早期减轻足细胞的损伤。结论:自噬发生在细胞凋亡之前,自噬介导了PAN诱导的足细胞凋亡。这些发现表明自噬可能成为未来治疗足细胞损伤和蛋白尿的新治疗靶点。
更新日期:2020-02-26
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