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Fibroblast growth factor receptor-like-1: a new therapeutic target and unfavorable prognostic indicator for rectal adenocarcinoma
Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-02-26 , DOI: 10.1080/10799893.2020.1731534
Ru-Zhen Jia 1 , Ji-Zhun Zhang 2 , Chang-Qing Jing 2 , Chen-Sheng Li 2 , Hong-Qing Zhuo 2
Affiliation  

Abstract Fibroblast growth factor receptor-like-1 (FGFRL1) is important to cell motility and links with tumorigenic potential in various types of cancers. To investigate the biological function and underlying mechanism of FGFRL1 in rectal adenocarcinoma, we conducted this study. TCGA and Oncomine databases were used to analyze FGFRL1 expression and its association with clinical characteristics or overall survival (OS) in rectal adenocarcinoma patients. siRNA strategy was implemented to knockdown FGFRL1 expression in rectal adenocarcinoma cells. CCK8, colony formation, wound healing, and transwell assays were implemented to measure cell behaviors. qRT-PCR and western blot were utilized to identify mRNA and protein expression levels. FGFRL1 was significantly increased in rectal adenocarcinoma tissue samples, either colon or rectum. High-regulation of FGFRL1 expression induced poorer outcome of rectal adenocarcinoma patients. Downregulation of FGFRL1 inhibited the proliferation, colony formation, migration, and invasion of SW837 cells. The MAPK pathway-related proteins, phosphorylation of MEK and ERK, were also decreased after si-FGFRL1 transfection. These findings demonstrated that FGFRL1, acting as a potential inducator, may promote the progression of rectal adenocarcinoma via activating the MAPK signaling pathway.

中文翻译:

成纤维细胞生长因子受体样1:直肠腺癌的新治疗靶点和不利预后指标

摘要 成纤维细胞生长因子受体样 1 (FGFRL1) 对细胞运动很重要,并与各种癌症的致瘤潜力有关。为了研究 FGFRL1 在直肠腺癌中的生物学功能和潜在机制,我们进行了这项研究。TCGA 和 Oncomine 数据库用于分析直肠腺癌患者的 FGFRL1 表达及其与临床特征或总生存期 (OS) 的关联。实施siRNA策略以抑制直肠腺癌细胞中的FGFRL1表达。实施 CCK8、集落形成、伤口愈合和 transwell 测定来测量细胞行为。qRT-PCR 和蛋白质印迹用于鉴定 mRNA 和蛋白质表达水平。FGFRL1 在直肠腺癌组织样本(结肠或直肠)中显着增加。FGFRL1 表达的高调节导致直肠腺癌患者的预后较差。FGFRL1 的下调抑制了 SW837 细胞的增殖、集落形成、迁移和侵袭。MAPK 通路相关蛋白、MEK 和 ERK 的磷酸化在 si-FGFRL1 转染后也降低。这些发现表明 FGFRL1 作为潜在的诱导物,可能通过激活 MAPK 信号通路促进直肠腺癌的进展。
更新日期:2020-02-26
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