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ABA-dependent K+ flux is one of the important features of the drought response that distinguishes Catalpa from two different habitats
Plant Signaling & Behavior ( IF 2.8 ) Pub Date : 2020-03-06 , DOI: 10.1080/15592324.2020.1735755
Wenjun Ma 1, 2, 3, 4 , Guijuan Yang 1, 2, 3, 4 , Yao Xiao 1, 2, 3, 4 , Xiyang Zhao 5 , Junhui Wang 1, 2, 3, 4
Affiliation  

ABSTRACT Abscisic acid (ABA)-induced stomatal closure can improve drought tolerance in higher plants. However, the relationship between ABA-related ion flux and improved drought resistance in the roots of woody plants is unclear. To investigate this relationship, we employed a noninvasive micro-test technique (NMT) to detect potassium (K+) flux in Catalpa fargesii and C. fargesii f. duclouxii after treatment with polyethylene glycol (PEG) and ABA. PEG treatment slightly increased the free proline content in both Catalpa species. However, simultaneous treatment with ABA and PEG resulted in a large increase in free proline content. Treatment with PEG led to a significant increase in K+ efflux, and both ABA and tetraethylammonium (TEA, a K+ channel inhibitor) blocked this efflux under short-term (1 d) and long-term (7 d) drought conditions. Furthermore, we detected SKOR (stelar K+ outward-rectifying channel) gene expression in roots, and the results showed that PEG significantly increased SKOR expression in C. fargesii f. duclouxii, but SKOR expression was inhibited by ABA in Catalpa fargesii. These findings indicate that ABA improves drought tolerance by inhibiting K+ efflux in Catalpa, but distinct ABA response patterns exist. Drought-tolerant species have better potassium retention are dependent on ABA, and can accumulate more proline than other species. SKOR is also ABA-dependent and sensitive to ABA, and K+ flux is a target of the ABA-mediated drought response.

中文翻译:

ABA 依赖性 K+ 通量是干旱响应的重要特征之一,可将楸树与两种不同的生境区分开来

摘要脱落酸(ABA)诱导的气孔关闭可以提高高等植物的耐旱性。然而,ABA相关离子通量与木本植物根部抗旱性提高之间的关系尚不清楚。为了研究这种关系,我们采用了一种无创微测试技术 (NMT) 来检测 Catalpa fargesii 和 C. fargesii f 中的钾 (K+) 通量。用聚乙二醇 (PEG) 和 ABA 处理后的 duclouxii。PEG 处理略微增加了两种楸属物种的游离脯氨酸含量。然而,同时用 ABA 和 PEG 处理导致游离脯氨酸含量大幅增加。PEG 处理导致 K+ 外流显着增加,ABA 和四乙铵(TEA,一种 K+ 通道抑制剂)在短期(1 天)和长期(7 天)干旱条件下均阻断了这种外流。此外,我们检测了根中 SKOR(恒星 K+ 外向整流通道)基因的表达,结果表明 PEG 显着增加了 C. fargesii f. 中的 SKOR 表达。duclouxii,但 ABA 在 Catalpa fargesii 中抑制了 SKOR 的表达。这些发现表明 ABA 通过抑制 Catalpa 中的 K+ 外流来提高耐旱性,但存在不同的 ABA 响应模式。耐旱物种具有更好的钾保留,依赖于 ABA,并且可以比其他物种积累更多的脯氨酸。SKOR 也依赖于 ABA 并且对 ABA 敏感,而 K+ 通量是 ABA 介导的干旱响应的目标。但 ABA 在楸树中抑制 SKOR 的表达。这些发现表明 ABA 通过抑制 Catalpa 中的 K+ 外流来提高耐旱性,但存在不同的 ABA 响应模式。耐旱物种具有更好的钾保留,依赖于 ABA,并且可以比其他物种积累更多的脯氨酸。SKOR 也依赖于 ABA 并且对 ABA 敏感,而 K+ 通量是 ABA 介导的干旱响应的目标。但 ABA 在楸树中抑制 SKOR 的表达。这些发现表明 ABA 通过抑制 Catalpa 中的 K+ 外流来提高耐旱性,但存在不同的 ABA 响应模式。耐旱物种具有更好的钾保留,依赖于 ABA,并且可以比其他物种积累更多的脯氨酸。SKOR 也依赖于 ABA 并且对 ABA 敏感,而 K+ 通量是 ABA 介导的干旱响应的目标。
更新日期:2020-03-06
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