Moluccella laevis L. (Lamiaceae) is an annual ornamental plant, naturally occurring in the areas of Western and Central Asia (Irano-Turanian area). It is a popular ornamental species, but its phytochemical profile remains poorly studied. This study presents the first report on in vitro cultures of M. laevis. The influence of two types of illumination and plant growth regulators (PGRs) supplementation on the morphogenetic response and the content of phenolic compounds were examined. Shoots were cultured on Murashige and Skoog (MS) solid basal medium. White light or photosynthetic active radiation (PAR) as well as three concentrations of 6-benzylaminopurine (BA) 2, 3 or 4 µM and one of the three auxins: 1-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA) or 4-amino-3,5,6-trichloropicolinic acid (picloram—PIC) at concentration of 0.5 µM were used for shoot proliferation. The highest number of axillary shoots per explant (3.4) was obtained for shoots grown on MS medium supplemented with 3-µM BA and 0.5-μM NAA under PAR light. However, the effect of PGRs treatment on shoot growth and development was stronger than the influence of light. The phytochemical analysis using Ultra-Performance Liquid Chromatography–Mass Spectrometry allowed identification of four hydroxycinnamic acids (caffeic, ferulic, rosmarinic, chlorogenic) and two cyanidin glycosides (cyanidin 3-O-galactoside, and cyanidin 3-O-malonylglucoside) in shoot clusters and callus tissue.

Moluccella laevis L.（唇形科）是一种一年生观赏植物，天然存在于西亚和中亚地区（伊朗-图兰人地区）。它是一种流行的观赏物种，但其植物化学特征仍然缺乏研究。这项研究提出了关于M. laevis体外培养的第一份报告。。检查了两种类型的光照和植物生长调节剂（PGR）补充对形态发生反应和酚类化合物含量的影响。在Murashige和Skoog（MS）固体基础培养基上培养芽。白光或光合作用活性辐射（PAR）以及三种浓度的2、3或4 µM的6-苄基氨基嘌呤（BA）和三种生长素之一：1-萘乙酸（NAA），吲哚-3-乙酸（IAA） ）或浓度为0.5 µM的4-氨基-3,5,6-三氯吡啶甲酸（picloram-PIC）用于芽增殖。在PAR光照下，在补充有3-μMBA和0.5-μMNAA的MS培养基上生长的枝条，可获得最高的每枝外植体枝条（3.4）。然而，PGRs处理对枝条生长和发育的影响强于光的影响。芽簇和愈伤组织中的O-半乳糖苷和花青素3- O-丙二酰葡萄糖苷）。