Ephedrannin B (EPB) has been shown to exert anti-inflammatory effects. However, the effect of EPB on respiratory syncytial virus infection (RSV) is not known. In this study, the cytotoxic effect of EPB was evaluated in BEAS-2B cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Reverse transcription quantitative polymerase chain reaction and Western blot assays were performed to determine the expression of target genes. The anti-viral effect of EPB was assessed by determining viral titers using plaque assay. We found that RSV infection caused a marked increase in interleukin (IL)-6, IL-8, IL-1β and tumor necrosis factor (TNF)-α production and release, which was concentration-dependently attenuated by EPB treatment. Furthermore, EPB decreased the expression of RSV fusion gene in RSV-infected BEAS-2B cells. Concomitantly, EPB treatment led to an obvious inhibition of viral replication in BEAS-2B cells. Besides, EPB suppressed RSV-induced mitogen-activated protein kinase/nuclear factor kappa-light-chain-enhancer of activated B cells signaling. In conclusion, EPB exerts anti-viral and anti-inflammatory properties in BEAS-2B cells infected with RSV.

中文翻译：

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麻黄素 B 在感染呼吸道合胞病毒的 BEAS-2B 细胞中发挥抗病毒和抗炎特性

麻黄素 B (EPB) 已被证明具有抗炎作用。然而，EPB 对呼吸道合胞病毒感染 (RSV) 的影响尚不清楚。在本研究中，使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物测定法在 BEAS-2B 细胞中评估了 EPB 的细胞毒性作用。进行逆转录定量聚合酶链反应和蛋白质印迹分析以确定靶基因的表达。EPB 的抗病毒作用是通过使用噬斑测定确定病毒滴度来评估的。我们发现 RSV 感染导致白细胞介素 (IL)-6、IL-8、IL-1β 和肿瘤坏死因子 (TNF)-α 的产生和释放显着增加，而 EPB 治疗会浓度依赖性地减弱这种情况。此外，EPB 降低了 RSV 感染的 BEAS-2B 细胞中 RSV 融合基因的表达。同时，EPB 处理导致 BEAS-2B 细胞中病毒复制的明显抑制。此外，EPB 抑制了 RSV 诱导的丝裂原活化蛋白激酶/核因子 kappa-轻链增强子的活化 B 细胞信号传导。总之，EPB 在感染 RSV 的 BEAS-2B 细胞中发挥抗病毒和抗炎特性。