Background

Glypicans play a role in the growth and metastasis of several human cancers. However, little is known about its function in thyroid cancer. Therefore, this study aimed to investigate the specific functions of glypican 2 (GPC2) in thyroid cancer cells.

Objective

The expression of six glypican family members (GPC1 to GPC6) was detected in different thyroid cancer cell lines. The abnormal expression of GPC2 was confirmed by immunohistochemistry in or around thyroid tumor tissues. It was overexpressed and silenced in highly (TPC1 and FTC-133) and poorly (ARO) differentiated thyroid cancer cell lines, respectively. Subsequently, its role in cell proliferation, apoptosis, migration, and invasion was investigated.

Results

We observed that almost all glypican family members were highly expressed in thyroid cancer cells, and GPC2 expression was higher in poorly differentiated thyroid cancer cell lines than highly differentiated cell lines. The knockdown of GPC2 inhibited cell proliferation, invasion, and migration and promoted apoptosis in the ARO cells, whereas GPC2 overexpression promoted cell proliferation, invasion, and migration and inhibited apoptosis in the TPC1 and FTC-133 cells.

Conclusions

GPC2 is highly expressed in thyroid cancer tissues and regulates cell viability, apoptosis, migration, and invasion.

中文翻译：

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Glypican 2调节甲状腺癌细胞的细胞增殖和转移

背景

升糖聚糖在几种人类癌症的生长和转移中发挥作用。然而，对其在甲状腺癌中的功能了解甚少。因此，本研究旨在研究Glypican 2（GPC2）在甲状腺癌细胞中的特定功能。

目的

在不同的甲状腺癌细胞系中检测到六个Glypican家族成员（GPC1至GPC6）的表达。通过免疫组织化学在甲状腺肿瘤组织中或周围证实了GPC2的异常表达。它分别在高分化（TPC1和FTC-133）和低分化（ARO）的甲状腺癌细胞系中过表达和沉默。随后，研究了其在细胞增殖，凋亡，迁移和侵袭中的作用。

结果

我们观察到几乎所有的Glypican家族成员都在甲状腺癌细胞中高表达，而GPC2在低分化甲状腺癌细胞系中的表达高于高分化细胞系。敲低GPC2抑制细胞增殖，侵袭和迁移并促进ARO细胞凋亡，而GPC2过表达促进TPC1和FTC-133细胞增殖，侵袭和迁移并抑制细胞凋亡。

结论

GPC2在甲状腺癌组织中高度表达，并调节细胞活力，凋亡，迁移和侵袭。