Backgrounds

miRNAs, about 20–25 bases in length, are short-chain non-coding RNAs existing in the body, which are widely involved in the biological regulation of the organism. miRNAs inhibit the expression of target genes by specifically binding to target genes, thereby exerting biological effects.

Methods

We investigated the function of miRNA-338-5p on inflammation and its possible mechanisms in colitis

Results

In DSS-induced colitis model, we found that miRNA-338-5p expression was reduced. Therefore, down-regulation of miRNA-338-5p increased inflammation and induced CXCR4, TXNIP, and NLRP3 protein expression in in vitro model. Meanwhile, over-expression of miRNA-338-5p reduced inflammation and suppressed CXCR4, TXNIP, and NLRP3 protein expression in in vitro model. Therefore, miRNA-338-5p may possess anti-inflammatory effects in colitis. However, si-CXCR4 reduced the anti-inflammatory effects of miRNA-338-5p in invitro model. Then, si-NLRP3 also reduced the anti-inflammatory effects of miRNA-338-5p in in vitro model. These results showed that miRNA-338-5p reduced inflammation in colitis through the TXNIP/NLRP3 inflammasome axis by CXCR4.

Conclusion

MiRNA-338-5p may potentially serve as novel therapeutic avenues for colitis.

中文翻译：

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MiRNA-338-5p在DSS诱发的结肠炎中通过CXCR4通过TXNIP / NLRP3炎性体轴减少炎症

背景资料

大约20-25个碱基长的miRNA是体内存在的短链非编码RNA，广泛参与了生物体的生物调节。miRNA通过与靶基因特异性结合来抑制靶基因的表达，从而发挥生物学作用。

方法

我们研究了miRNA-338-5p对炎症的作用及其在结肠炎中的可能机制

结果

在DSS诱导的结肠炎模型中，我们发现miRNA-338-5p表达降低。因此，miRNA-338-5p的下调增加了炎症，并在体外模型中诱导了CXCR4，TXNIP和NLRP3蛋白的表达。同时，在体外模型中，miRNA-338-5p的过表达减少炎症并抑制CXCR4，TXNIP和NLRP3蛋白表达。因此，miRNA-338-5p在结肠炎中可能具有抗炎作用。但是，si-CXCR4在体外模型中降低了miRNA-338-5p的抗炎作用。然后，si-NLRP3在体外模型中也降低了miRNA-338-5p的抗炎作用。这些结果表明，miRNA-338-5p通过CXCR4通过TXNIP / NLRP3炎性体轴减少了结肠炎的炎症。

结论

MiRNA-338-5p可能会成为结肠炎的新型治疗途径。